Screening and Identification of Keratinase-producing Endophyte

doi:10.7506/spkx1002-6630-201117047

Abstract

Abstract: A strain that can degrade native feather in 48 h was isolated from camphor endophytes through primary screening on skim milk plates and secondary screening based on degradation of steamed chicken feather and native chicken feather after liquid shake flask cultivation. The isolated strain was identified as Bacillus cereus Z-3 based on morphological observation, biochemical experiments and 16S rDNA sequence analysis. Keratinase from Bacillus cereus Z-3 had the most powerful ability to degrade chicken feather, followed by wool and hair.

Key words: endophyte, keratinase, Bacillus cereus, screening, identification

CLC Number:

TS201.3

DU Yong-xin，SHENG Ji-ping，CHENG Fan-sheng，WANG Zheng-rong，SHEN Lin. Screening and Identification of Keratinase-producing Endophyte[J]. FOOD SCIENCE, 2011, 32(17): 230-233.

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Screening and Identification of Keratinase-producing Endophyte

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