Separation and Purification of Proanthocyanidin from Grape Seeds by Vibratory Shear Enhanced Membrane Filtration

doi:10.7506/spkx1002-6630-201206002

Abstract

Abstract: Objective: To investigate the effectiveness of vibratory shear enhanced membrane filtration technology in separating and purifying proanthocyanidin from grape seeds. Method: Membranes with different pore sizes were used to microfiltrate and concentrate grape seed extract. Membrane flux was determined as a measure of membrane stability. The removal of unwanted impurities was evaluated by weighing. Proanthocyanidin content was assayed by Folin-Ciocalteu method. Results: Vibratory shear enhanced membrane filtration effectively removed macromolecular impurities from grape seed extract, consequently increasing the purity of proanthocyanidin, and allowed continuous separation and concentration with few operations, high efficiency and low cost when used in combination with concentration membrane. Conclusions: Vibratory shear enhanced membrane filtration is applicable for the separation and purification of proanthocyanidin from grape seeds.

Key words: vibratory shear enhanced membrane filtration, Folin-Ciocalteau method, proanthocyanidin, separation and purification

CLC Number:

TS201.1

LUJun-wen，LIRong，LIANGYong-hong. Separation and Purification of Proanthocyanidin from Grape Seeds by Vibratory Shear Enhanced Membrane Filtration[J]. FOOD SCIENCE, 2012, 33(6): 7-10.

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Separation and Purification of Proanthocyanidin from Grape Seeds by Vibratory Shear Enhanced Membrane Filtration

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