Feasibility Study on Triterpenoids Production using Supercritical Fluid Extraction and Simulated Moving Bed Chromatography

Abstract

Abstract: Triterpenoids in the fruiting body of Ganoderma Lucidum was extracted by supercritical carbon dioxide extraction (SFE). Simulated Moving Bed (SMB), a continuous chromatography was then applied to remove the impurities from the crude extract. The contents of Ganoderma lucidum A (GAA), Ganoderma F (GAF) and Ganoderma B (GLB) was monitored during the process. In this study, the extraction conditions for triterpenoids were optimized. Using different SMB column configurations for different mobile phases, the hydrophilic and lyophilic impurities in the crude extract were effectively removed, and the total content of triterpenoids was effectively increased. The results showed that the extraction yield of triterpenoids in the fruiting body could be sufficiently improved by adding ethanol as co-solvent. Triterpenoids can be thoroughly extracted in two hours and the crude extract contains GAA 4.50%, GAF 3.39% and GLB 0.29%. Followed by SMB separation with 1-1-3/3 column configuration, it is observed that hydrophilic impurities can be effectively removed. The content of the indicators increases and reaches 19.34% for GAA, 15.51% for GAF, and 0.74% for GLB. While SMB separation with 2/3/3 column configuration, lyophilic impurities can be removed. The content of the indicators reaches 5.70% for GAA, 4.17% for GAF, and 0.85% for GLB. Therefore, the removal of hydrophilic impurities from the crude extract should be the main task to increase the content of triterpenoids in the product.

Key words: Supercritical Fluid, Simulated Moving Bed, Triterpenoids, Separation &Purification

CLC Number:

TS218

References

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