FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (12): 233-237.doi: 10.7506/spkx1002-6630-201212047

• Analysis & Detection • Previous Articles     Next Articles

Determination of Total and Individual Phenolics in Potato Flesh and Skin from Different Growing Areas

CAI Li-chuang1,2,LUO Cheng-yao1,LUO Xiao-yan2,RUAN Zheng1,*,WEN Hong-yan1   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, China)
  • Online:2012-06-25 Published:2012-07-27

Abstract: The contents of total and individual phenolics in potato flesh and skin from different growing areas in Xinjiang, Jiangxi, Fujian and Beijing were comparatively determined by both HPLC and Folin-Ciocalteu colorimetry. The gradient elution of phenolics was carried out on YMC-C18 column (250 mm × 4.6 mm, 5 μm) using methanol (mobile phase A) and .5% phosphoric acid (mobile phase B). Baseline separation was observed for chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid and rutin. All of them showed good linear relationship over a wide concentration range. The average spike recoveries for them from a known sample were 91.36%-102.23% (n = 3). The total phenolic contents in all samples under investigation were 0.238-1.190 mg/g. Chlorogenic acid accounted for 80.59%-89.06% of the total phenolics in potato flesh compared to 65.54%-80.00% in potato skin. The HPLC method was quick, accurate, reliable and repeatable. The Folin-Ciocalteu colorimetry method was more suitable for the determination of total phenolics in potato.

Key words: potato, phenolic compounds, high performance liquid chromatography, folin method

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