FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (15): 121-128.doi: 10.7506/spkx1002-6630-20200729-365

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Food-Grade κ-Carrageenan on Risk of Colitis and Accumulation of Body Fat in Obese Mice

ZHANG Hui, YANG Ruili, LIU Fang, ZHOU Sainan, LU Na, TANG Qingjuan   

  1. (College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China)
  • Online:2021-08-15 Published:2021-08-27

Abstract: Objective: To explore the effect of various dosage of food-grade κ-carrageenan on the risk of colitis and the accumulation of body fat in obese mice. Methods: After feeding C57BL/6Cnc male mice with a low-fat or high-fat diet for 5 weeks, the mice fed with the high-fat diet was 22% heavier than those fed with the low-fat diet, indicating that an animal of obesity model was successfully constructed. The mice were divided into seven groups, including normal control (NC), high-fat diet (HFD) model and HFD + 0.05% (H0.05%), 0.5% (H0.5%), 1% (H1%), 2.5% (H2.5%) or 5% (H5%) carrageenan groups. After feeding for 6 weeks, the mice were sacrificed to examine indicators related to colon inflammation and body fat accumulation. Results: Disease activity index (DAI), myeloperoxidase activity and the relative mRNA expression of inflammatory factors in the colon, and colonic physiological structure were not significantly different among all groups (P > 0.05). The secretion of prostaglandin E2 in the colon of mice in the H1% and H2.5% groups decreased by 13.94% and 20.75%, respectively compared to the HFD group (P < 0.05). The body mass, body fat percentage and epididymal adipocyte size of mice in the H5% group decreased by 10.51%, 37.67% and 31.59%, respectively compared with the HFD group (P < 0.05). Conclusion: Dietary addition of 0.05%–5% food-grade κ-carrageenan had no risk of inducing colitis in obese mice, and 5% carrageenan inhibited the accumulation of body fat.

Key words: food-grade carrageenan; dosage; obese; colitis; body fat accumulation

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