Epigallocatechin Gallate (EGCG) Regulates Lipid Droplet Accumulation in 3T3-L1 Adipocytes through Activating Adenosine Monophosphate-Activated Protein Kinase (AMPK) and Consequently Inhibiting Peroxisome Proliferator Activated Receptor-γ (PPARγ)

doi:10.7506/spkx1002-6630-20201221-234

FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (23): 152-161.doi: 10.7506/spkx1002-6630-20201221-234

• Nutrition & Hygiene • Previous Articles Next Articles

Epigallocatechin Gallate (EGCG) Regulates Lipid Droplet Accumulation in 3T3-L1 Adipocytes through Activating Adenosine Monophosphate-Activated Protein Kinase (AMPK) and Consequently Inhibiting Peroxisome Proliferator Activated Receptor-γ (PPARγ)

GUAN Qiaoli, WU Xiaoyun, LI Fang, SHENG Jun

(1. Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China; 2. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 3. College of Science, Yunnan Agricultural University, Kunming 650201, China)

Online:2021-12-15 Published:2021-12-29

Abstract

Abstract: In this study, we explored the effect of epigallocatechin gallate (EGCG) on lipid droplet accumulation in mature 3T3-L1 adipocytes. For this purpose, 3T3-L1 preadipocytes were induced to differentiate?into mature adipocytes. After EGCG treatment, the size of lipid droplets formed in the adipocytes decreased in a time and concentration-dependent manner. On the other hand, EGCG didn’t lead to cell apoptosis. Compared with the control group, EGCG treatment significantly down-regulated the mRNA expression levels of the genes associated with lipid metabolism, transport and absorption. EGCG treatment also significantly decreased the expression of peroxisome proliferator-activated receptor-γ (PPARγ) and activated adenosine monophosphate (AMP)-activated protein kinase (AMPK). EGCG up-regulated the expression of phospho-AMPK and inhibited the expression of PPARγ in mature adipocytes further treated with the AMPK inhibitor dorsomorphin. Therefore, EGCG can reduce lipid droplet accumulation in mature 3T3-L1 adipocytes by activating AMPK, consequently inhibiting PPARγ expression, and reducing the mRNA expression of the genes related to lipid metabolism, transport and absorption.

Key words: epigallocatechin gallate; 3T3-L1 adipocytes; AMP-activated protein kinase; peroxisome proliferators-activated receptors; lipid droplet accumulation

CLC Number:

TS201.4

GUAN Qiaoli, WU Xiaoyun, LI Fang, SHENG Jun. Epigallocatechin Gallate (EGCG) Regulates Lipid Droplet Accumulation in 3T3-L1 Adipocytes through Activating Adenosine Monophosphate-Activated Protein Kinase (AMPK) and Consequently Inhibiting Peroxisome Proliferator Activated Receptor-γ (PPARγ)[J]. FOOD SCIENCE, 2021, 42(23): 152-161.

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Epigallocatechin Gallate (EGCG) Regulates Lipid Droplet Accumulation in 3T3-L1 Adipocytes through Activating Adenosine Monophosphate-Activated Protein Kinase (AMPK) and Consequently Inhibiting Peroxisome Proliferator Activated Receptor-γ (PPARγ)

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