Anti-inflammatory Effect of Camellia fascicularis Polyphenols Based on Zebrafish Model and Network Pharmacology

Abstract

Abstract: To investigate the molecular mechanism of Camellia fascicularis polyphenols (CFP) exerting anti-inflammatory effects, the anti-inflammatory activity of CFP was evaluated by zebrafish model and the components of CFP were analyzed using ultra-performance liquid chromatography combined with quadrupole-time-of-flight mass/mass spectrometry (UPLC-Q-TOF-MS/MS). Then, the targets of effective ingredients and inflammation were predicted through SwissTargetPrediction and DisGeNET databases. The protein-protein interaction (PPI), gene ontology (GO) function and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were achieved with STRING and DAVID databases. Furthermore, molecular docking and visualization of key components and core proteins were performed using AutoDockTools and PyMOL. The results showed that 21 phenolic compounds were identified from CFP and 32 intersection targets were screened. The GO function and KEGG pathway enrichment analysis indicated that CFP exerted anti-inflammatory effect by acting on the core targets as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), prostaglandin-endoperoxide synthase 2 (PTGS2) and matrix metallopeptidase 2 (MMP2) through the biological processes of positive regulation of cell proliferation, cytoplasm, adenosine triphosphate (ATP) binding, and by further regulating signaling pathways like arachidonic acid metabolism, hypoxia inducible factor-1 (HIF-1) signaling pathway and platelet activation. Molecular docking results demonstrated excellent binding activity existed between the key components in CFP and inflammation targets. The research could provide a theoretical basis for the development of anti-inflammatory products with polyphenol components of C. fascicularis.

Key words: Camellia fascicularis, inflammation, zebrafish model, network pharmacology, molecular docking

CLC Number:

R285.5

TANG Junrong KAN Huan ZHAO Ping ZHANG Yingjun Guiliang Zhang Yun Liu. Anti-inflammatory Effect of Camellia fascicularis Polyphenols Based on Zebrafish Model and Network Pharmacology[J]. FOOD SCIENCE, 0, (): 0-0.

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