Isolation,purification and structural characterization of Semen astragali complanati polysaccharides

Abstract

Abstract: polysaccharides was extracted from Semen Astragali complanati (SACP) by hot water and ethanol precipitation, and then ethanol fractional precipitation was applied to separate the polysaccharide fractions SACP-25. Their physicochemical properties were determined by colorimetric method, and the molecular weight distribution and monosaccharide composition was analyzed by size exclusion chromatography coupled with muti-angle laser light scattering and high-performance anion exchange chromatography coupled with pulsed amperometric detector. Results indicated that SACP-25 was a high-purified fraction, with total sugar content of 87.40% and composed of galactose (42.92%) and mannose (57.08%), and it showed random coil in 0.1 mol/L NaNO3. Methylation combined with gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy analysis revealed that the main chain of SACP-25 was composed of β-1,4-linked-Manp with Galp side chains attached to C-6 of the Manp residues. This studyindicated that Semen A. complanati could be chosen as good natural source of galactomannans, that is a kind of water-soluble dietary fiber. This study also added research support for the relative ordered structure of polysaccharides theory. Meanwhile, it would also provide theoretical foundation for the development and application of SACP in functional foods and pharmaceutical industries.

Key words: Semen astragali complanati, Polysaccharides, Galactomanans, Chemical structure

CLC Number:

TS201.2

Shao-Ping NIE. Isolation,purification and structural characterization of Semen astragali complanati polysaccharides[J]. FOOD SCIENCE, 0, (): 0-0.

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