Effect and mechanism of near-freezing temperature on delaying postharvest Dendrocalamus brandisii shoots lignification based on transcriptome analysis

Abstract

Abstract: Abstract: In order to investigate the effect and relevant mechanism of NFT (near-freezing temperature) on delaying bamboo shoots lignification, with D.brandisii (Dendrocalamus brandisii) shoots as material, the preservation effect of NFT on D.brandisii shoots was summarized based on the changes of hardness, respiration intensity, weight loss and lignin contents during storage; in addition, the relevant mechanism was hypothesized based on the change of PAL, C4H, CAD and POD activities and the expression of genes which are key in lignin synthesization. It was indicated that NFT effectively delayed the postharvest senescence of D.brandisii shoots, which inhibited the increase of hardness, respiratory strength and weight loss of D.brandisii shoots, maintained the lignin content of D.brandisii shoots. Furthermore, it was found that NFT stabilized PAL, CAD, C4H, and POD activities. Moreover, 58 differentially expressed genes in the phenylpropane metabolic pathway were screened based on transcriptome technology, of which 33 genes were down-regulated by NFT; among them, the transcription of PAL, 4CL, HCT, CCR, CAD, was roughly reduced by NFT. It was hypothesized that NFT might delay the postharvest senescence and maintain lignin content of D.brandisii shoots by stabilizing the activities of PAL, CAD, C4H and POD and down-regulating gene expression in the phenylpropane metabolic pathway including PAL, 4CL and HCT genes, etc.

Key words: Keywords: Dendrocalamus brandisii shoots, near-freezing temperature, lignification, transcriptome, postharvest

CLC Number:

TS255.3

Shuang-He YANG Hai-Bo LUO. Effect and mechanism of near-freezing temperature on delaying postharvest Dendrocalamus brandisii shoots lignification based on transcriptome analysis[J]. FOOD SCIENCE.

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