Abstract:

The optimum condition of jellyfish (Rhopilema esculentum Kiahinouye 1891) collagen was studied, and the effect of jellyfish collagen on promoting the wound healing was studied based on the model of mouse skin wounds. Experimental factors and their levels were determined by single-factor tests, and then a three factors and three levels test was designed based on the principle of Box-Benhnken design. The extraction conditions were optimized through response surface method (RSM). In the experiment of wound healing in mouse, cutting-skin injures model in mouse were created and wounds were treated with collagen, at the same time yunnan baiyao was used as positive control. The size of wounds was measured through cross measurement, and the healing rates were calculated. Results showed the optimal conditions of jellyfish collagen extraction were obtained as following: extraction time 62.64 h, the solid/water ratio of 1:23.6, acetic acid concentration of 0.562 mol/L. Animal tests showed that treatment with collagen could significantly accelerate the process of re-epithelialization, and promote wound healing.

Key words: jellyfish, collagen, Response surface method (RSM), wound healing