Ethanol Extraction of Puerarin by Cellulase Hydrolysis

doi:10.7506/spkx1002-6630-201024009

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 46-49.doi: 10.7506/spkx1002-6630-201024009

• Processing Technology • Previous Articles Next Articles

Ethanol Extraction of Puerarin by Cellulase Hydrolysis

WANG Xing-min1,2,3，YIN Zhong-yi1,3，LI Xin1，ZHANG Yu-wen1

1. School of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067,
China；2. School of Resources and Environmental Science; Chongqing University, Chongqing 400033, China；3. Engineering
Research Centre for Waste Oil Recovery Technology and Equipment, Ministry of Education, Chongqing Technology and Business
University, Chongqing 400067, China

Received:2010-09-21 Revised:2010-11-13 Online:2010-12-25 Published:2010-12-29
Contact: WANG Xing-min1 E-mail:wxm0826@ctbu.edu.cn

Abstract

Abstract:

Puerarin was extracted by enzymatic hydrolysis with microbe and cellulase from waste pueraria. High performance liquid chromatography was used to determine puerarin content for exploring the effects of enzymatic hydrolysis time, enzyme addition amount, pH and material-liquid ratio as well as cross-interaction on extraction rate of puerarin. Results indicated that pueraria amount of 5 g, cellulose concentration of 40% compared with pueraria weight, pueraria-water ratio of 1:10, enzymatic hydrolysis pH of 5, enzymatic hydrolysis temperature of 30 ℃, enzymatic hydrolysis time of 36 h and ethanol extraction time of 3 h. Under the optimal enzymatic hydrolysis and extraction conditions, the yield of puerarin was up to 33.54 mg. These investigations will provide a theoretical reference for the exploration and utilization of pueraria.

Key words: cellulase, puerarin, pueraria waste, enzymolysis, biological interaction

CLC Number:

TQ 028.8

WANG Xing-min1,2,3，YIN Zhong-yi1,3，LI Xin1，ZHANG Yu-wen1. Ethanol Extraction of Puerarin by Cellulase Hydrolysis[J]. FOOD SCIENCE, 2010, 31(24): 46-49.

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Ethanol Extraction of Puerarin by Cellulase Hydrolysis

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