Abstract:

Objective: To establish a determination method for hyperin in Rosa davurica Pall.. Methods: A HPLC method was developed using Shim-pack VP-ODS C18 (4.6 mm × 150 mm, 5 μm) column as the stationary phase and CH3OH-0.2% H3PO4 as the mobile phase. The flow rate of mobile phase, injection volume of the sample and the column temperature were 1.0 mL/min, 20 μL and 40 ℃, respectively. The UV detection wavelength was 360 nm. Results: A good linear relationship between integral value of peak area and hyperin concentration in the range of 4.76－76.16 μg/mL (r = 0.9993) was observed. The average recovery rate of hyperin was 98.06% with the RSD of 1.72%. Conclusion: The HPLC determination of hyperin in Rosa davurica Pall. was characteristics of convenience and accuracy, which can be used for the quality control of Rosa davurica Pall.

Key words: Rosa davurica Pall., hyperin, HPLC method