Abstract:

Adinandra nitida is a traditional Chinese herb for health drink, of which leaves are rich in flavonoids. The optimization of total flavonoids extraction from the leaves of Adinandra nitida with ethanol as the extraction solvent was conducted in this work, which was also aimed at examining if the total flavonoids from the leaves of Adinandra nitida have the ability to inhibit the proliferation of lung cancer A549 cells. The optimal extraction conditions of total flavonoids from Adinandra nitida leaves were determined by response surface methodology to be: extraction time, 10 min; ethanol concentration, 63%, extraction temperature, 70 ℃; and material/liquid ratio, 1:20. The extraction rate of total flavonoids was (95.58 ± 0.49) % under the optimal extraction conditions. The inhibitory effect of the total flavonoids from the leaves of Adinandra nitida on the proliferation of lung cancer A549 cells was evaluated by MTT assay. The total flavonoids could significantly inhibit the proliferation of lung cancer A549 cells in significant time-dependent and dose-dependent manners.

Key words: Adinandra nitida, total flavonoids, extraction condition, physiological function