FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 375-377.doi: 10.7506/spkx1002-6630-201022082

• Analysis & Detection • Previous Articles     Next Articles

Determination of 2-Keto-D-gluconic Acid in Fermentation Broth by Polarimetry

SUN Wen-jing1,ZHOU Yan-zheng1,FENG Xiao-yan2,WEI Zhuan3,YU Si-lian4,ZHOU Qiang4   

  1. (1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
    2. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;
    3. Department of Pharmaceutical, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China;
    4. Parchn Sodium Isovitamin C Co. Ltd., Dexing 334221, China)
  • Received:2010-05-07 Online:2010-11-25 Published:2010-12-29
  • Contact: SUN Wen-jing E-mail:sunwenjing1919@163.com

Abstract:

The 2-keto-D-gluconic acid in fermentation broth was calculated on the basis of glucose concentration determined by optical rotation degree. The application feasibility of polarimetry was also investigated. Results indicated that a good linear relationship between optical rotation degree and the concentration of 2-keto-D-gluconic acid and glucose (R2 > 0.9999). The average recovery rate was 102.60% with RSD of 2.17%. This method has characteristics of precision, stability and reproducibility. Therefore, it can be used for determining 2-keto-D-gluconic acid in fermentation broth.

Key words: 2-keto-D-gluconic acid, glucose, fermentation broth, polarimetry, determination

CLC Number: