FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 312-315.doi: 10.7506/spkx1002-6630-201021070

• Bioengineering • Previous Articles     Next Articles

Isolation, Identification and Fermentation Analysis of a Cellulose-degrading Bacterium from Arctium lappa L. Rhizosphere

HOU Jin-hui,CAI Kan,ZHENG Bao-gang,CAO Yu-peng,YE Jun   

  1. Food Engineering (Bioengineering) Department, Xuzhou Institute of Technology, Xuzhou 221008, China
  • Received:2010-07-02 Online:2010-11-15 Published:2010-12-29
  • Contact: HOU Jin-hui E-mail:houjinhui0@126.com

Abstract:

In this study, a cellulose-degrading bacterium, NP10, was isolated from burdock (Arctium lappa L.) rhizosphere. NP10 was identified to belong to the family of Bacillus sp. and its 16S rDNA Genbank accession number was FJ908093. Meanwhile, NP10 was identified as an alkaline cellulose-degrading strain through batch fermentation. A maximum filter paper activity of 18.2 U/mL was obtained after 48 h fermentation under the conditions of 32 ℃ and initial pH 8, and the optimal fermentation temperature and duration as well as initial pH were 32 ℃, 36 h and 7, respectively, for achieving a maximum carboxymethyl cellulose activity of 8.1 U/mL.

Key words: burdock, rhizosphere, cellulose-degrading strain, Bacillus

CLC Number: