Determination of the Content of Vitamin C in Siraitia grosvenorii Fruits by HPLC

doi:10.7506/spkx1002-6630-201020064

Abstract

Abstract:

Objective: To set up a HPLC method for the determination of the content of vitamin C in the fruits of Siraitia grosvenorii. Methods: The chromatographic separation was achieved on SinoChrom ODS-BP (250 mm×4.6 mm, 5μm) held at 25 ℃ using a mixture composed of 0.05% H3PO4 and CH3OH (98:2, V/V) as the mobile phase at a flow rate of 1.0 mL/min. The diode array detector wavelength was set at 242 nm. The injection volume used was 10μL. Results: The developed calibration curve of vitamin C displayed good linearity over the concentration range from 0.08 to 5.0 mg/mL, with a correlation coefficient equaling 1. The average recovery of five replicates for vitamin C was 99.3% with 1.93% relative standard deviation (RSD). Conclusion: This method is simple, quick, precise and stable, and can used for determining the content of vitamin C in the fruits of Siraitia grosvenorii.

Key words: Siraitia grosvenorii, HPLC, VC

CLC Number:

Q946.6

NING De-sheng，LIANG Xiao-yan，FANG Hong. Determination of the Content of Vitamin C in Siraitia grosvenorii Fruits by HPLC[J]. FOOD SCIENCE, 2010, 31(20): 311-313.

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Determination of the Content of Vitamin C in Siraitia grosvenorii Fruits by HPLC

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