Application of Cell Culture Model to Antioxidant Activity Analysis of an Ethanol Extract from Propolis

doi:10.7506/spkx1002-6630-201019041

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (19): 190-193.doi: 10.7506/spkx1002-6630-201019041

• Basic Research • Previous Articles Next Articles

Application of Cell Culture Model to Antioxidant Activity Analysis of an Ethanol Extract from Propolis

WU Zheng-shuang1,2，DONG Jie2，ZHANG Hong-cheng2，ZHAO Liang-liang3，GAO Wen-hong1,*

1. College of Light Industry and Food, South China University of Technology, Guangzhou 510641, China；
2. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China；
3. College of Food Engineering, Harbin University of Commerce, Harbin 150028, China

Received:2010-06-01 Online:2010-10-15 Published:2010-12-29
Contact: GAO Wen-hong E-mail:zzhc@sohu.com

Abstract

Abstract:

A cellular antioxidant activity (CAA) assay for quantifying the antioxidant activity of an ethanol extract from propolis (EEP) provided by Bee Research Institute, Chinese Academy of Agricultural Sciences has been developed. Dichlorofluoresceindiacetate (DCFH-DA) as a probe, which is easily oxidized into fluorescent dichlorofluorescein (DCF) by reactive oxygen species (ROS), was trapped within cells and the method was used to measure the ability of EEP to prevent the formation of DCF by 2,2′-azobis(2-amidinopropane) dihydrochloride (ABAP)-generated peroxyl radicals in human liver carcinoma HepG2 cells with multi-functional microplate reader and inverted fluorescence microscope. The decrease in cellular fluorescence intensity compared to the control cells indicates the antioxidant capacity of EEP. In addition, this bioactivity depended on dose. The concentration of EEP corresponding to CAA unit50 was only 0.14 mg/mL. A ROS clearance rate of 47.05% was obtained at an EEP concentration of 0.5 mg/mL.

Key words: human liver carcinoma HepG2 cells, reactive oxygen species, cellular antioxidant activity

CLC Number:

S896

WU Zheng-shuang1,2，DONG Jie2，ZHANG Hong-cheng2，ZHAO Liang-liang3，GAO Wen-hong1,*. Application of Cell Culture Model to Antioxidant Activity Analysis of an Ethanol Extract from Propolis[J]. FOOD SCIENCE, 2010, 31(19): 190-193.

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Application of Cell Culture Model to Antioxidant Activity Analysis of an Ethanol Extract from Propolis

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