Effect of Polysaccharides from Ganoderma atrum on Spleen Lymphocyte Proliferation and Induction of Cytokine in Mice

doi:10.7506/spkx1002-6630-201019077

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (19): 351-354.doi: 10.7506/spkx1002-6630-201019077

• Nutrition & Hygiene • Previous Articles Next Articles

Effect of Polysaccharides from Ganoderma atrum on Spleen Lymphocyte Proliferation and Induction of Cytokine in Mice

ZHU Ke-xue，NIE Shao-ping，LI Wen-juan，YU Qiang，ZHANG Shen-shen，XIE Ming-yong*

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2010-06-24 Revised:2010-09-12 Online:2010-10-15 Published:2010-12-29
Contact: XIE Ming-yong E-mail:myxie@ncu.edu.cn

Abstract

Abstract:

Objective: To investigate the effect of polysaccharides from Ganoderma atrum (PSG-1) on spleen lymphocyte proliferation and induction of cytokine in mice. Methods: The effects of treatments with PSG-1 and with PSG-1 containing motigen at different concentrations on spleen lymphocyte proliferation were determined by MTT. The contents of IL-2 and TNF-α were determined by using ELISA. Results: PSG-1 at a concentration ranging from 20 to160 μg/mL resulted in a significant increase of lymphocyte proliferation, and 48 h administration gave the best proliferation. PSG-1 could also enhance the effect of Con A-induced T lymphocyte proliferation and the stimulation index of LPS-induced B lymphocytes. Moreover, PSG-1 could significantly increase the secretion of IL-2 and TNF-α in spleen lymphocyte. Conclusion: PSG-1 can effectively increase lymphocyte proliferation and enhance the effect of Con A, LPS-induced T and B cell growth. PSG-1 has the ability to enhance immune responses by increasing the secretion of IL-2 and TNF-α.

Key words: Ganoderma atrum polysaccharides, spleen lymphocyte, MTT, ELISA, IL-2, TNF- α

CLC Number:

ZHU Ke-xue，NIE Shao-ping，LI Wen-juan，YU Qiang，ZHANG Shen-shen，XIE Ming-yong*. Effect of Polysaccharides from Ganoderma atrum on Spleen Lymphocyte Proliferation and Induction of Cytokine in Mice[J]. FOOD SCIENCE, 2010, 31(19): 351-354.

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Effect of Polysaccharides from Ganoderma atrum on Spleen Lymphocyte Proliferation and Induction of Cytokine in Mice

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