Comparative Study on the Effects of Lactoferrin and Lactoferricin from Different Sources on Proliferation of Mouse Spleen Lymphocytes

doi:10.7506/spkx1002-6630-201813027

Abstract

Abstract: The effects of lactoferrin from bovine whey (LFW), lactoferrin from bovine colostrum (LFC), human lactoferrin (LFH), human lactoferricin (LfcinH) and bovine lactoferricin (LfcinB) on the proliferation of spleen lymphocytes from mice were studied, and different lactoferrin (LF) and lactoferricin (Lfcin) were compared. The cell counting kit-8 assay was used to measure cell proliferation after 24, 48 and 72 h of culture. Moreover, the effects of different LF and Lfcin combined with mitogen on the proliferation of mouse T and B lymphocytes were also evaluated. The results showed that both LF and Lfcin had the most significant effect in promoting the proliferation of spleen lymphocytes at 48 h, which was concentration-dependent in a certain range. Both LF and LFcin enhanced T lymphocyte proliferation induced by concanavalin A and B lymphocyte proliferation induced by lipopolysaccharide in a certain concentration range. These findings indicated that the two types of bovine lactoferrins (LFB) as well as Lfcin can substitute LFH in infant formula products to enhance immune function in infants and young children, and the recommended concentration for LFB and Lfcin was 0.50–2.00 mg/mL and 75–125 μg/mL, respectively.

Key words: lactoferrin, lactoferricin, spleen lymphocyte, proliferation

CLC Number:

TS252.1

PI Bingbing, ZHAO Xiao, Lü Pingping, PENG Yukai, PAN Lin, XU Xiaoxi. Comparative Study on the Effects of Lactoferrin and Lactoferricin from Different Sources on Proliferation of Mouse Spleen Lymphocytes[J]. FOOD SCIENCE, 2018, 39(13): 184-189.

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Comparative Study on the Effects of Lactoferrin and Lactoferricin from Different Sources on Proliferation of Mouse Spleen Lymphocytes

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