Abstract:

Objective: To develop an optimal process for hyperin extraction from Acanthopana sessiliflorus (Rupr.et Maxim.) leaves. Methods: The major factors affecting hyperin extraction yield were ethanol concentration, extraction time and liquid-tosolid ratio. They were optimized using central composite design and response surface methodology. Multiple linear regression and quadratic polynomial fitting of experimental data obtained from central composite design were carried out to set up a mathematical model with hyperin extraction yield as a function of the above factors. Their optimal values were chosen using ridge analysis and verified experimentally. Results: The optimal conditions for the extraction of hyperin were found to be: ethanol concentration 69.80%, liquid-to-solid ratio 23.17:1 and extraction time 86.56 min. Conclusion: Central composite design and response surface methodology are suitable for optimizing the extraction of hyperin from Acanthopana sessiliflorus leaves. This method is simple, convenient and accurate.

Key words: Acanthopanax sessiliflorus, central composite design, response surface methodology, hyperin, optimization