Abstract:

Resveratrol liposomes were prepared using ethanol injection method and solid resveratrol liposomes were obtained after lyophilization. The effects of core material-to-wall material ratio, emulsifier type and amount, amount of mannitol as a cryoprotectant, oil phase-to-water phase ratio on entrapment efficiency were investigated in single factor experiments. The optimal preparation conditions of resveratrol liposome were as follows: lecithin-to-cholesterol-to-resveratrol ratio 10:2:1, amount of Tween-80 used as emulsifier 0.2%, amount of mannitol 2%, and oil phase-to-water phase ratio 1:10.The entrapment efficiency of solid resveratrol liposomes prepared under these optimum conditions was 84.68%. Therefore, ethanol injection method for preparing resveratrol liposome can provide higher entrapment efficiency and feasibility.

Key words: esveratrol, solid liposome, preparation condition, ethanol injection method