Abstract:

Objective: To analyze the effects of three different administration routes of Spirulina platensis polysaccharides on 24 cytokines in mouse serum, followed by understanding of the immunoregulatory effect of Spirulina platensis polysaccharides. Methods: Three administration routes used were oral administration, intraperitoneal injection and intravenous injection at different doses each. Mice orally administered the same volume of normal saline were served as a control group. Serum was separated from eye ball at 3 h after administration for oral administration and at 2 h after administration for intraperitoneal injection and intravenous injection. The contents of 24 cytokines in mouse serum were examined using Milliplex map kit or luminex xMAP technology. Results: Compared with the control group, each treatment group showed significantly higher serum IL-1α, IL-1β, IL-6, TNF-α and IFN-γ contents, indicating that Spirulina platensis polysaccharides have an anxo-action on inflammation cytokines. Among the three administration routes, the effect of intraperitoneal injection on cytokines was the most obvious, and the cell signaling mode of this route mainly consisted of NF-κB，MAPK and JAK/STAT. Conclusion: In general, Spirulina platensis polysaccharides have immunopromoting effect on cytokines, and their immunoregulatory and multiple physiological functions may be exerted through interactions among intestinal mucous membrane receptors and exciting the secretion of cytokines by homologous cells.

Key words: Spirulina platensis polysaccharides, cytokine, immunoregulatory effect