Isolation, Purification and Biological Activity Assessment of an Antimicrobial Protein from Marine Paenibacillus polymyxa Strain L1-9

doi:10.7506/spkx1002-6630-201017075

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (17): 335-339.doi: 10.7506/spkx1002-6630-201017075

• Bioengineering • Previous Articles Next Articles

Isolation, Purification and Biological Activity Assessment of an Antimicrobial Protein from Marine Paenibacillus polymyxa Strain L1-9

MA Gui-zhen1,2，WANG Shu-fang1，BAO Zeng-hai1,2，WU Shao-jie1，XIA Zhen-qiang1，LI Shi-dong3

1. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China；2. Jiangsu
Marine Resources Development Research Institute, Lianyungang 222005, China；3. Plant Protection Institute of the Chinese
Academy of Agricultural Sciences, Beijing 100081, China

Received:2010-06-30 Revised:2010-08-31 Online:2010-09-15 Published:2010-12-29
Contact: MA Gui-zhen E-mail:guizhenma@sohu.com,malan2@sina.com

Abstract

Abstract:

An antimicrobial protein was obtained from the fermentation broth of a Paenibacillus polymyxa strain isolated from the sea area near Lianyungang (numbered L1-9), which has antagonic effect against a variety of pathogenic fungi, after ammonium sulfate precipitation and DEAE Sepharose Fast Flow ion exchange and Sephacryl G-200 filtration chromatographic separation. This protein was found to have the ability to inhibit the growth of Staphyloccocus aureus and the mycelial growth and spore germination of Helminthosporium sativum and possess a molecular weight of about 31 kD.

Key words: marine Paenibacillus polymyxa strain L1-9, antimicrobial protein, purification

CLC Number:

Q815

MA Gui-zhen1,2，WANG Shu-fang1，BAO Zeng-hai1,2，WU Shao-jie1，XIA Zhen-qiang1，LI Shi-dong3. Isolation, Purification and Biological Activity Assessment of an Antimicrobial Protein from Marine Paenibacillus polymyxa Strain L1-9[J]. FOOD SCIENCE, 2010, 31(17): 335-339.

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Isolation, Purification and Biological Activity Assessment of an Antimicrobial Protein from Marine Paenibacillus polymyxa Strain L1-9

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