Abstract:

An optimal macroporous resin and its optimal technological parameters for purification of crude total flavonoids separated from Bergenia emeiensis CY Wu leaves were investigated in the present study. HPD-600 resin was chosen due to its excellent adsorption and desorption performance. Static and dynamic adsorption and desorption behaviors of HPD-600 resin were evaluated for achieving an optimal purification of total flavonoids. Results showed that the optimal purification process was that sample solution at pH 3－4 and the concentration level of 0.5－0.6 mg/mL was made to flow through HPD-600 resin column with a maximum injection volume of 3 BV and subsequently, the column was washed with 3 BV of water for the removal of impurities and eluted with 4 BV of 90% ethanol at a flow rate of 2 mL/min. More than 80% total flavonoids with a purity of more than 29% were recovered using the above process. These data demonstrate the excellence of HPD-600 resin in purifying total flavonoids from Bergenia emeiensis CY Wu leaves and the advantages of the developed method for industrial application, like simple operation, safety, and low cost.

Key words: macroporous adsorption resin, B. emeiensis leaves, total flavonoids, purification