食品科学 ›› 2010, Vol. 31 ›› Issue (20): 52-57.doi: 10.7506/spkx1002-6630-201020011

• 工艺技术 • 上一篇    下一篇

蛋白酶预处理对玉米蛋白粉中叶黄素提取的影响

周海胜,蒋凌志,黄 梅*   

  1. 浙江大学化学工程与生物工程学系
  • 收稿日期:2010-01-08 修回日期:2010-06-12 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 黄梅 E-mail:huangm@zju.edu.cn

Effect of Proteolytic Pre-treatment on the Extraction of Xanthophylls from Corn Gluten Meal

ZHOU Hai-sheng,JIANG Ling-zhi,HUANG Mei*   

  1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China
  • Received:2010-01-08 Revised:2010-06-12 Online:2010-10-25 Published:2010-12-29
  • Contact: HUANG Mei E-mail:huangm@zju.edu.cn

摘要:

选用玉米蛋白粉为原料,通过蛋白酶预处理再进行有机溶剂提取的方式制备叶黄素。与玉米蛋白粉中叶黄素的非酶解直接提取法相比,原料玉米蛋白粉采用最佳蛋白酶水解工艺预处理后(中性蛋白酶,酶用量为14000U/g玉米蛋白粉,液固比为20:1(mL/g),温度40℃,水解时间5h),不但使叶黄素的提取量由非酶解直接提取的165.3μg/g 提高到340.5μg/g,而且浸提时间由5h 缩短为20min。

关键词: 叶黄素, 玉米蛋白粉, 蛋白酶水解, 提取

Abstract:

After being pre-treated with protease, corn gluten meal received organic solvent extraction to obtain xanthophylls. Neutral protease was selected out of three commercially available proteases to pretreat corn gluten meal due to the highest extraction efficiency of xanthophylls, and methanol was found to be the best solvent for the extraction of xanthophylls among the ten solvents tested. The optimal conditions for neutral protease hydrolysis were determined to be: enzyme load, 14000 U/g substrate, liquid/solid ratio, 20:1; reaction temperature 40 ℃; and reaction duration, 5 h. Compared with direct extraction without proteolytic pre-treatment, the optimized neutral protease hydrolysis resulted in an increase of extraction efficiency of xanthophylls from 165.3 to 340.5 μg/g and a reduction of extraction duration from 5 hour to 20 min.

Key words: xanthophylls, corn gluten meal, proteolytic hydrolysis, extraction

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