普洱茶茶色素对HepG2细胞脂质代谢的影响及作用机理

doi:10.7506/spkx1002-6630-201717033

食品科学 ›› 2017, Vol. 38 ›› Issue (17): 203-209.doi: 10.7506/spkx1002-6630-201717033

普洱茶茶色素对HepG2细胞脂质代谢的影响及作用机理

陈亚蓝1，王雪青1,*，王怡雯1，郑子晴1，叶美霞1，付芳芳1，赵培1，宋文军1，王素英1，白晓丽2，李长文2

1.天津商业大学生物技术与食品科学学院，天津市食品与生物技术重点实验室，天津 300134；2.云南天士力帝泊洱生物茶集团有限公司，云南普洱 665100

出版日期:2017-09-15 发布日期:2017-09-12
基金资助:
天津市高等学校创新团队建设规划资助项目（TD12-5049）；天津市高校科技发展基金计划项目（20120603）；天津商业大学大学生研究训练计划（SRT）基金项目

Effect and Mechanism of Pigments from Pu-erh Tea on Lipid Metabolism in HepG2 Cell Model

CHEN Yalan1, WANG Xueqing1,*, WANG Yiwen1, ZHENG Ziqing1, YE Meixia1, FU Fangfang1, ZHAO Pei1, SONG Wenjun1,WANG Suying1, BAI Xiaoli2, LI Changwen2

1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,Tianjin 300134, China; 2. Yunnan Tasly Deepure Biological Tea Group Co. Ltd., Puer 665100, China

Online:2017-09-15 Published:2017-09-12

摘要/Abstract

摘要： 以正常培养和油酸诱导培养的人肝癌细胞系（human hepatocellular liver carcinoma cell line，HepG2）细胞为模型，通过测定普洱茶茶色素对HepG2细胞内甘油三酯（triglyceride，TG）和总胆固醇（total cholesterol，TC）含量，细胞中脂肪酸合成酶（fatty acid synthase，FAS）、固醇调节元件结合蛋白1c（sterol regulatory elementbinding protein 1c，SREBP-1c）、三磷酸腺苷结合转运子A1（ATP binding cassette transporter A1，ABCA1）、胆固醇7α-羟化酶（cholesterol 7α-hydroxylase，CYP7A1）的转录水平，磷酸化腺苷酸活化蛋白激酶（phospho-AMPactivatedprotein kinase，p-AMPK）的蛋白表达水平研究普洱茶茶色素的减肥降脂作用机制。结果显示，普洱茶茶色素能明显降低油酸诱导的HepG2细胞模型中TG和TC含量，作用程度依赖于普洱茶的作用质量浓度。但对正常培养的HepG2的TG、TC作用影响不显著。经过普洱茶茶色素作用油酸诱导HepG2细胞24 h后，能显著下调细胞的FAS和SREBP-1c的mRNA表达水平（P＜0.05），显著上调ABCA1的转录水平（P＜0.05），且使CYP7A1的转录水平呈上升趋势，并显著上调p-AMPK蛋白的表达量（P＜0.05）。因此，普洱茶茶色素可通过调控上述调控因子和酶的表达而改善油酸诱导下HepG2细胞的脂质代谢水平。

关键词: HepG2, 脂质代谢, 普洱茶, 色素

Abstract: The contents of triglyceride (TG) and total cholesterol (TG), the mRNA expression level of fatty acid synthase (FAS), sterol regulatory element binding protein 1c (SREBP-1c), cholesterol 7α-hydroxylase (CYP7A1) and ATP binding cassette transporter A1 (ABCA1), and the protein expression level of phospho-AMP-activated protein kinase (p-AMPK) were investigated for studying the effects of pigments from Pu-erh tea on lipid metabolism in HepG2 cell line cultured with normal or oleic acid enriched medium. The results showed that pigments from Pu-erh tea significantly decreased the contents of TG and TC in HepG2 cells cultured in oleic acid-enriched medium in a dose-dependent manner, although having no significant effect on HepG2 cells cultured in normal medium. The mRNA expression levels of FAS and SREBP-1c were significantly down-regulated, and the mRNA level of ABCA1 was significantly up-regulated, the mRNA expression level of CYP7A1 showed an increased tendency and the protein expression level of p-AMPK was significantly up-regulated after the treatment with pigments from Pu-erh tea for 24 hours in HepG2 cells cultured in oleic acid-enriched medium (P < 0.05). Therefore, pigments from Pu-erh tea could improve lipid metabolism in HepG2 cells cultured in oleic acid-enriched medium by regulating the mRNA levels of enzymes and transcription factors.

Key words: HepG2, lipid metabolism, Pu-erh tea, pigments

中图分类号:

R961

陈亚蓝，王雪青，王怡雯，郑子晴，叶美霞，付芳芳，赵培，宋文军，王素英，白晓丽，李长文. 普洱茶茶色素对HepG2细胞脂质代谢的影响及作用机理[J]. 食品科学, 2017, 38(17): 203-209.

CHEN Yalan, WANG Xueqing, WANG Yiwen, ZHENG Ziqing, YE Meixia, FU Fangfang, ZHAO Pei, SONG Wenjun,WANG Suying, BAI Xiaoli, LI Changwen. Effect and Mechanism of Pigments from Pu-erh Tea on Lipid Metabolism in HepG2 Cell Model[J]. FOOD SCIENCE, 2017, 38(17): 203-209.

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普洱茶茶色素对HepG2细胞脂质代谢的影响及作用机理

Effect and Mechanism of Pigments from Pu-erh Tea on Lipid Metabolism in HepG2 Cell Model

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