食品科学 ›› 2017, Vol. 38 ›› Issue (6): 130-135.doi: 10.7506/spkx1002-6630-201706020

• 生物工程 • 上一篇    下一篇

山药低聚糖对2 种双歧杆菌的体外促生长作用

刘 露,张 雁,池建伟,魏振承,张名位   

  1. 1.广东省农业科学院蚕业与农产品加工研究所,农业部功能食品重点实验室,广东省农产品加工重点实验室,广东 广州 510610;2.华中农业大学食品科学技术学院,湖北 武汉 430070;3.广东丸美生物技术股份有限公司,广东 广州 510530
  • 出版日期:2017-03-25 发布日期:2017-03-28
  • 基金资助:
    广东省工业高新技术项目(2013B010102011);广州市产学研协同创新重大专项(201508020036);广东省省属科研机构改革创新领域项目(2016B070701012);广东省应用型科技研发专项(2015B020230005)

Promoting Effects of Oligosaccharides from Chinese Yam on the Growth of Two Species of Bifidobacteria in Vitro

LIU Lu, ZHANG Yan, CHI Jianwei, WEI Zhencheng, ZHANG Mingwei   

  1. 1. Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China; 2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 3. Guangdong Marubi Biotechnology Co. Ltd., Guangzhou 510530, China
  • Online:2017-03-25 Published:2017-03-28

摘要: 利用体外模型探讨了山药低聚糖对青春双歧杆菌和动物双歧杆菌的生长促进作用。以山药低聚糖取代葡萄糖为碳源添加到双歧杆菌基础培养基中,发现山药低聚糖对青春双歧杆菌和动物双歧杆菌的体外生长具有显著促进作用:随着山药低聚糖的加入,培养液中活菌数增加,pH值降低。当山药低聚糖取代葡萄糖且质量分数为0.5%时,青春双歧杆菌和动物双歧杆菌的最大生长量分别提高了(10.70±4.93)%和(6.02±2.00)%;pH值分别下降(2.64±0.001)%和(3.85±0.65)%。同时,山药低聚糖可提高青春双歧杆菌和动物双歧杆菌的生长速率,使其生长提前进入稳定期。

关键词: 山药, 低聚糖, 双歧杆菌, 促生长作用, 益生元

Abstract: In the present work, the growth-promoting effects of Chinese yam oligosaccharides on Bifidobacterium adolescentis and Bifidobacterium animals in vitro were examined. Being used as a substitute of carbon source for glucose in culture medium, Chinese yam oligosaccharides had significant promoting effects on the growth of bifidobacteria. With the increase in added Chinese yam oligosaccharides, the viable count of the probiotic bacteria increased, while the pH value decreased. By glucose substitution with 0.5% Chinese yam oligosaccharides, the viable counts of B. adolescentis and B. animals increased by (10.70 ± 4.93)% and (6.02 ± 2.00)%, respectively, and the pH value decreased by (2.64 ± 0.001)% and (3.85 ± 0.65)% respectively. Meanwhile, Chinese yam oligosaccharides promoted the growth rate of bifidobacteria and resulted in earlier onset of the stationary growth phase.

Key words: Chinese yam, oligosaccharides, Bifidobacterium, growth-promoting effects, prebiotics

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