关键词: 豌豆纤维粉, 膳食纤维, 低聚糖, 抗氧化性

Abstract: In this study, pea fiber powder was used as a starting material to prepare oligosaccharides by enzymatic hydrolysis. In order to determine the optimal ranges of process parameters, the effects of solid-to-solid ratio, xylanase dosage, cellulase dosage and reaction temperature on the contents of oligosaccharides, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity and hydroxyl radical scavenging capacity were investigated using a quadratic rotatable composite design. The results indicated that a solid-to-solid ratio of 1:20.7–1:24.5 (g/mL), a xylanase dosage of 170.9–176.7 U/g, a cellulase dosage of 316.6–320.4 U/g and a reaction temperature of 52.1–57.6 ℃ were found to be the optimal conditions to obtain higher oligosaccharide content of 13.98%–15.02%, higher DPPH radical scavenging capacity of 30.56%–33.21% and higher hydroxyl radical scavenging capacity of 39.36%–42.44%. Analysis by liquid chromatography showed that the oligosaccharide prepared under the optimized conditions consisted mainly of arabinose, glucose, galactose, cellobiose, cellotriose and cellotetraose.

Key words: pea fiber powder, dietary fiber, oligosaccharides, antioxidant activity