关键词: 山蕗菜根, 多糖, 提取工艺, 响应曲面法优化

Abstract:

Crude polysaccharides were prepared from the residue of Petasites tricholobus roots after flavonoids extraction. According to single-factor experiments, three levels of the factors including extraction temperature, extraction time and liquid/solid ratio were selected for the central composite design to establish a quadric regression equation for predicting the yield of polysaccharides. By response surface analysis, the optimum extraction conditions were obtained as follows: extraction temperature 70 ℃, extraction time 2.3 h and liquid/solid ratio 45:1, which led to estimated and observed values of maximal yield of polysaccharides of 35.04% and 34.74%, respectively.

Key words: Petasites tricholobus roots, polysaccharides, extraction technology, response surface methodology