食品科学 ›› 2009, Vol. 30 ›› Issue (23 ): 131-134.doi: 10.7506/spkx1002-6300-200923028

• 基础研究 • 上一篇    下一篇

黄伞菌丝体多糖的分离鉴定及其抗氧化性研究

杨立红,黄清荣,冯培勇,蔡德华,狄慧玲   

  1. 鲁东大学生命科学学院
  • 收稿日期:2009-06-14 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 杨立红 E-mail:Yanglihong33@263.net
  • 基金资助:

    山东省科技厅、财政厅良种工程项目(鲁科农字[2006]90 号);鲁东大学学科建设资助项目

Isolation, Identification and Anti-oxidant Activity of Polysaccharides from Pholiota adiposa Mycelium

YANG Li-hong,HUANG Qing-rong,FENG Pei-yong,CAI De-hua,DI Hui-ling   

  1. College of Life Science, Ludong University, Yantai 264025, China
  • Received:2009-06-14 Online:2009-12-01 Published:2010-12-29
  • Contact: YANG Li-hong E-mail:Yanglihong33@263.net

摘要:

黄伞菌丝体烘干后经热水提取,乙醇沉淀得粗多糖。粗多糖经Sevag 法除蛋白后DEAE- 纤维素柱层析,分离得到两种多糖组分PAMP Ⅰ和PAMP Ⅱ。红外光谱分析表明PAMP Ⅰ和PAMP Ⅱ均为含有葡萄糖醛酸的β-D-吡喃葡聚糖。采用化学比色法测定黄伞菌丝体多糖在体外的总抗氧化能力、抑制活性超氧阴离子自由基的能力、清除羟自由基的能力及对小鼠肝组织中脂质过氧化产物丙二醛(MDA)含量的的影响。结果表明:一定浓度范围内不同纯度的黄伞菌丝体多糖均有总抗氧化能力,能清除O2·和·OH 及抑制MDA 的生成,且呈现一定剂量关系。表明黄伞菌丝体多糖在一定浓度范围内具有抗氧化作用。

关键词: 黄伞菌丝体, 多糖, 抗氧化性

Abstract:

Crude polysaccharides were obtained from Pholiota adiposa Mycelium through hot-water extraction and ethanol precipitation. Crude polysaccharides after removing protein through Sevage method were purified using DEAE-cellulose column chromatography to obtain two polysaccharide ingredients, designated as PAMP-I and PAMP-II. Glucuronic acid β-D-pyranglucan was identified in both purified polysacchride components through IR spectrum analysis. Total anti-oxidant capability, scavenging capability to superoxide anion and hydroxyl free radicals, and effect of polysaccharides from Pholiota adiposa Mycelium on MDA content were evaluated using colorimetry. Results indicated that polysaccharides from Pholiota adiposa Mycelium had anti-oxidant capability such as scavenging free radicals and suppressing MDA generation in a dose-dependent mode.

Key words: Pholiota adiposa Mycelium, polysaccharide, anti-oxidant capability

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