油菜蜂花粉及其提取物对大鼠酒精性肝损伤组织学影响

食品科学 ›› 2008, Vol. 29 ›› Issue (7): 442-444.

油菜蜂花粉及其提取物对大鼠酒精性肝损伤组织学影响

孙丽萍,王大仟,廖磊,彭文君

中国农业科学院蜜蜂研究所；北京市卫生局临床药学研究所；中国农业科学院蜜蜂研究所北京100093；北京100035；北京100093

出版日期:2008-07-15 发布日期:2011-07-28

Effects of Rape Pollen and Its Extracts on Histology of Rat with Alcoholic Liver Injury

SUN Li-Ping, WANG Da-Qian, LIAO Lei, PENG Wen-Jun

1.Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 2.Institute of Beijing Health Administration Clinical Pharmacy, Beijing 100035, China

Online:2008-07-15 Published:2011-07-28

摘要/Abstract

摘要： 观察油菜蜂花粉及其提取物对大鼠酒精性肝损伤病理组织学的影响,为蜂花粉在酒精性肝损伤防治领域的应用提供实验依据,本实验采用大鼠白酒灌胃造模;以正常饮食、饮水组为对照;以花粉及其提取物治疗后,于各组大鼠肝脏取材,常规HE染色、光镜观察。结果显示模型组肝正常组织结构消失,肝细胞水样变,部分细胞可见脂肪变,肝细胞出现多处灶状坏死,单核、淋巴细胞浸润;经过花粉及其提取物治疗,肝组织损伤有不同程度的减轻,其中提取物乙酸乙酯组、正丁醇组和蒸馏水组的肝损伤程度明显减轻,肝脏损害最轻微,多数肝细胞结构清晰,接近正常状态,没有明显的病理变化。实验表明油菜蜂花粉及其提取物对大鼠酒精性肝损伤具有保护作用,其中提取物乙酸乙酯组、正丁醇组和蒸馏水组作用显著。

关键词: 酒精性肝损伤, 花粉及其提取物, 组织学

Abstract: In order to provide foundation for application of rape pollen in control of alcoholic liver injury, effects of rape pollen and its extracts on pathohistology of rat with alcoholic liver injury were studied. The rats were administered orally distillate spirit to induce alcoholic liver injury. Control group could take food and water freely. After therapy with rape pollen and its extracts, livers of rats were isolated. The liver slices were dyed by routine HE and observed by light microscopy. In model group, normal structure of liver of model group rat disappears, hepatic cell shows obvious hydopic and fatty change and focus necrosis, and lymphocyte and lymphomonocyte infiltrate. In therapy group, damage of liver shows extenuation at different degrees. The liver damage treated by acetoacetate, n-butanol or distilled water extracts shows significant extenuation with clear structure of liver tissue without obvious pathological change. These experimental results indicated that rape pollen and its extracts have protective effects on alcoholic liver injury of rat. The effects of its acetoacetate, n-butanol and distilled water extracts are obvious.

Key words: alcoholic liver injury, rape pollen, histology

孙丽萍, 王大仟, 廖磊, 彭文君. 油菜蜂花粉及其提取物对大鼠酒精性肝损伤组织学影响[J]. 食品科学, 2008, 29(7): 442-444.

SUN Li-Ping, WANG Da-Qian, LIAO Lei, PENG Wen-Jun. Effects of Rape Pollen and Its Extracts on Histology of Rat with Alcoholic Liver Injury[J]. FOOD SCIENCE, 2008, 29(7): 442-444.

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