食品科学 ›› 2008, Vol. 29 ›› Issue (2): 247-251.

• 生物工程 • 上一篇    下一篇

生物合成法生产β-胡萝卜素发酵条件研究

 戴德慧, 胡伟莲, 吕圭源, 李巍   

  1. 浙江中医药大学药学院; 浙江科技学院生物与化学工程学院; 浙江中医药大学药学院 浙江杭州310053 浙江科技学院生物与化学工程学院; 浙江杭州310012; 浙江杭州310053;
  • 出版日期:2008-02-15 发布日期:2011-08-24

Study on Fermentation Conditions of β-carotene by Biosynthesis

 DAI  De-Hui, HU  Wei-Lian, 吕Gui-Yuan , LI  Wei   

  1. 1.College of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China;2.Department of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310012, China
  • Online:2008-02-15 Published:2011-08-24

摘要: 对三孢布拉氏霉菌生物合成β-胡萝卜素的发酵条件进行了研究。在一系列单因素试验的基础上,初步得到β-胡萝卜素合成的发酵条件,分析了各因素对β-胡萝卜素产量及发酵生物量进行了分析。经正交设计优化后,得到三孢布拉氏霉菌的最佳发酵条件为:发酵温度为28℃,初始pH为7.0,正负菌种接种比例1:1,接种量为6%,发酵时间为156~162h,种龄42h,在此优化的发酵条件下,三孢布拉氏霉菌的β-胡萝卜素产量为772.47mg/L,比优化前提高了117.3%。

关键词: 三孢布拉氏霉菌, &beta, -胡萝卜素, 生物合成, 正交设计

Abstract:  The elementary fermentation conditions was obtained through a series of single factor tests. Based on the analysis of these results, the optimal culture conditions were confirmed by orthogonal test. The results showed that the optimum conditions are culture temperature 28 ℃, original pH 7.0, the ratio of strain(+) to strain (-)1:1, inoculum size 6%, and culture time 156 ~162 h. On the optimum conditions, the yield of β-carotene increased by 117.3% compared with the original fermentation conditions, up to 772.47 mg/L, which pave the groundwork for future industrialization.

Key words: Blakeslea trispora; &beta, -carotene; biosynthesis; orthogonal design ..;