食品科学 ›› 2007, Vol. 28 ›› Issue (7): 145-148.

• 工艺技术 • 上一篇    下一篇

羽衣甘蓝叶黄素提取纯化研究

 王向东, 赵国建   

  1. 山西师范大学食品科学与工程系; 山西师范大学食品科学与工程系 山西临汾041000; 山西临汾041000;
  • 出版日期:2007-07-15 发布日期:2011-10-24

Research on Extraction and Purification of Lutein from Brassic oleraced var. acephala DC.

 WANG  Xiang-Dong, ZHAO  Guo-Jian   

  1. Department of Food Science and Engineering, Shanxi Normal University, Linfen 041000, China
  • Online:2007-07-15 Published:2011-10-24

摘要: 以羽衣甘蓝冻干粉末为原料,采用THF浸提(10:1)、10%KOH-C2H5OH溶液皂化(6.5:1)的方法,研究提取羽衣甘蓝叶黄素的最佳条件;采用重结晶法纯化叶黄素粗品,通过HPLC测定纯度。结果表明,在40Hz超声波作用下,提取温度30℃、提取时间20min,叶黄素的提取量最高,达到3289.42μg/100g;游离态叶黄素纯度达到88.13%。

关键词: 叶黄素, 羽衣甘蓝, 超声波, 提取, 纯化

Abstract: The aim of this study is to determine the best conditions extracting lutein from Brassic oleraced var. acephala DC., based on its dried powder being soaked in THF(10:1) and saponified through 10% KOH-C2H5OH (6.5:1). Raw lutein was purified through recrystallization and its purity was measured with HPLC. The results showed that under the circumstances of 40Hz ultrasonic wave, the extracted lutein rate reached 3289.42 μg/100 g with the extracted temperature 30 ℃ and the extracted time 20 min. The purity of lutein in free state reached 88.13%.

Key words: lutein, Brassic oleraced var. acephala DC., ultrasonic wave, extraction, purification