蒲公英绿原酸提取分离工艺的研究

食品科学 ›› 2006, Vol. 27 ›› Issue (7): 140-144.

蒲公英绿原酸提取分离工艺的研究

沈奇, 赵厚民, 张卫明, 陆长梅, 吴国荣

南京师范大学生命科学学院；江苏省理化测试中心；南京野生植物综合利用研究院；

出版日期:2006-07-15 发布日期:2011-09-29

Study on Extraction and Separation Technology of Chlorogenic Acid in Dandelion

SHEN Qi, ZHAO Hou-Min, ZHANG Wei-Ming, LU Chang-Mei, Wu-Guo-Rong

1.College of Life Science, Nanjing Normal University, Nanjing 210097, China; 2.Jiangsu Center of Physics and Chemistry Test, Nanjing 210042, China; 3.Institute of Comprehensive Utilization of Wild Plant, Nanjing 210042, China

Online:2006-07-15 Published:2011-09-29

摘要/Abstract

摘要： 本文主要采用了正交分析的方法研究了从蒲公英中提取绿原酸,及采用大孔树脂NKA-9进一步分离绿原酸的最适条件,并采用制备性液相色谱的方法将其纯化。确定从蒲公英中提取绿原酸的最佳工艺参数为:物料比1:16,乙醇浓度80%,温度60℃,反应时间2h,绿原酸提取率可达60%以上。选用NKA-9树脂来分离绿原酸,确定其最适的分离条件是:上样浓度为0.26mg/ml,pH2.0,进样液体积/大孔树脂质量比值为12,流速为2ml/min,洗脱剂30%乙醇,绿原酸提取物纯度可达25.3%。最后采用半制备性液相色谱分离得绿原酸提取物,其纯度达到87.6%。

关键词: 蒲公英, 绿原酸, 提取, 正交试验, 分离

Abstract: Conditions of extraction and further isolation of chlorogenic acid from dandelion (Taraxacum. mongolicum Hand) in orthogonal test with macroporous resin NKA-9 were studied in this paper. Chlorogenic acid was isolated from dandelion with semi-preparing liquid chromatography. The optimum extraction conditions for dandelion chlorogenic acid were established as follows: ratio of material to liquor 1:16, 80% ethanol(V/V) as solvent and reflux extraction at 60℃ for 2 hours. The extraction rate of chlorogenic acid is as high as 69%. Chlorogenic acid was purified with macroporous resin NKA-9. The optimum purified conditions were established as follows: the sample concerntration 0.26mg/ml (pH 2.0), the ratio of the sample volume to the mass of macroporous resin NKA-9 12, and 30% ethanol as eluting solvent at 2ml/min. the purity of obtained chlorogenic acid is 25.3%. Finally, chlorogenic acid of higher purification (87.6%) is obtained with semi-preparing liquid chromatography.

Key words: dandelion, chlorogenic acid, abstraction, orthogonal test, separation

沈奇, 赵厚民, 张卫明, 陆长梅, 吴国荣. 蒲公英绿原酸提取分离工艺的研究[J]. 食品科学, 2006, 27(7): 140-144.

SHEN Qi, ZHAO Hou-Min, ZHANG Wei-Ming, LU Chang-Mei, Wu-Guo-Rong. Study on Extraction and Separation Technology of Chlorogenic Acid in Dandelion[J]. FOOD SCIENCE, 2006, 27(7): 140-144.

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