食品科学 ›› 2009, Vol. 30 ›› Issue (19 ): 263-267.doi: 10.7506/spkx1002-6630-200919061

• 生物工程 • 上一篇    下一篇

Viili中乳酸菌的分离、鉴定及其产胞外多糖的初步研究

郭 亮1,谭强来1,万翠香1,徐 锋1,魏 华1,*,曾 明2   

  1. 1.南昌大学 食品科学与技术国家重点实验室 2. 中国药品生物制品检定所
  • 收稿日期:2009-06-09 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 魏 华 E-mail:hua_wei114@yahoo.com.cn
  • 基金资助:

    国家“973”重点及计划项目(2005DKA21202);江西省自然科学基金项目(0630143)

Isolation, Identification and Exopolysaccharide-producing Ability of Lactic Acid Bacteria from Viili

GUO Liang1 TAN Qiang-lai1 WAN Cui-xiang1 XU Feng1 WEI Hua1,* ZENG Ming2   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
    2. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China
  • Received:2009-06-09 Online:2009-10-01 Published:2010-12-29
  • Contact: WEI Hua E-mail:hua_wei114@yahoo.com.cn

摘要:

从Viili 中分离得到两株具有较高产胞外多糖能力的乳酸菌,其产多糖量可达111.7mg/L和106.5mg/L。对菌株I、菌株II进行16S rDNA序列分析及生理生化鉴定。16S rDNA序列测序和API 50 CHL 试纸条鉴定结果表明:两株菌分别为Lactobacillus paracasei ssp. paracasei和Lactobacillus delbrueckii ssp. bulgaricus。

关键词: Viili, 胞外多糖, 乳酸菌, 16S rDNA

Abstract:

Two exopolysaccharide (EPS)-producing lactic acid bacterial isolates (strains I and II) isolated from viili were identified by 16S rDNA sequence alignment and biochemical and physiological identification with API 50 CHL kits and their EPS-producing abilities were studied. Strains Ⅰand Ⅱ were identified as Lactobacillus paracasei ssp. paracasei and Lactobacillus delbrueckii ssp. bulgaricus, and the EPS yields in skimmed milk fermented by them were 111.7 and 106.5 mg/L, respectively.

Key words: viili, exopolysaccharide, lactic acid bacteria, 16S rDNA

中图分类号: