食品科学 ›› 2006, Vol. 27 ›› Issue (10): 292-296.

• 工艺技术 • 上一篇    下一篇

酶法提取麒麟菜膳食纤维工艺的研究

 李来好,  杨少玲,  戚勃   

  1. 中国水产科学研究院南海水产研究所;广东海洋大学食品科技学院
  • 出版日期:2006-10-15 发布日期:2011-11-16

Studies of Optimization of Extraction Process of Dietery Fibre from Eucheuma with Biologic Enzyme by Using Uniform Design

 LI  Lai-Hao,   Yang-Shao-Ling,   Qi-Bo   

  1. 1.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;2.College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524025, China
  • Online:2006-10-15 Published:2011-11-16

摘要: 分别采用正交和均匀设计法,优选出麒麟菜(Eucheuma)膳食纤维的最佳漂白和提取工艺条件,并对提取的膳食纤维进行了分析。结果表明:最佳漂白条件——漂白液浓度2g/L、pH7.0、漂白时间40min;最佳提取条件——加水量4%(L/g)、煮沸时间60min、在60~65℃下分别加入0.15%的蛋白酶和0.1%的α-淀粉酶、分别酶解60和30min。在此条件下,提取率为39.06%,膨胀力为38.6ml/g、持水力为2045.8%,膳食纤维含有较低的蛋白质、脂肪且无淀粉检出,具有较高含量的Ca、P、K等矿物元素,其功能指标优于化学法提取的麒麟菜膳食纤维和小麦麸皮标准膳食纤维,有望在人体中发挥重要的生理功效。

关键词: 均匀设计, 麒麟菜, 膳食纤维, 提取

Abstract: The optimal conditions of bleaching and extracting of dietary fibre(DF) from Eucheuma were adopted by usingorthogonal and uniform design,and the dietary fibre were analysed. The results showed that bleaching with a concentration of 2g/ L bleacher under pH7.0 for 30min gaved the better results, and the extracting with a 4% (water: Eucheuma, L/g) ratio at 100 ℃ for 60min, and then added 0.15% protease and 0.1% α-amylase at 60~65℃ for 60min and 30min respectively gaved the best results. Under the optimal bleaching and extracting condition, the extracting ratio was 39.06%, the swollen volume was 38.6ml/ g, the water holding capacity was 2045.8%, the content of protein and fat were lower with no starch, but contained higher mineral element—Ca, P and K. The functional indexes above were higher than standard dietary fibre—wheat bran and dietary fibre from Eucheuma extracted chemically, suggesting that dietary fibre from Eucheuma bring into play a larger physiological efficacy on body.

Key words:  , uniform design; Eucheuma; dietary fibre; extract;