食品科学 ›› 2006, Vol. 27 ›› Issue (10): 354-358.

• 工艺技术 • 上一篇    下一篇

油菜花粉中谷蛋白的最佳提取工艺研究

 胡筱波, 罗祖友, 吴谋成, 李娟, 李孚杰   

  1. 华中农业大学食品科技学院; 湖北民族学院生物科学与技术学院;
  • 出版日期:2006-10-15 发布日期:2011-11-16

Study on the Extraction of Glutelins of Rape Pollen

 HU  Xiao-Bo, LUO  Zu-You, WU  Mou-Cheng, LI  Juan, LI  Fu-Jie   

  1. 1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2.Department of Biological Science and Technology, Hubei Institute for Nationalities, Enshi 445000, China
  • Online:2006-10-15 Published:2011-11-16

摘要:  本文研究了油菜花粉中的蛋白质组分分布以及碱浓度、pH值、浸提时间和温度对油菜花粉中碱溶谷蛋白提取率的影响,并在此基础上确定了谷蛋白的最佳提取条件。研究结果表明,破壁脱脂后的油菜花粉中蛋白质组分分布为清蛋白34.66%,球蛋白1.930%,醇溶蛋白2.810%,碱溶谷蛋白49.56%,复合蛋白11.04%,其中主要是清蛋白和谷蛋白;谷蛋白的最佳提取条件是料液比为1:20,氢氧化钠浓度0.30%,pH值为11,浸提时间75min,温度为70℃。

关键词: 油菜花粉, 谷蛋白, 提取

Abstract: Study was conducted on the effect of protein composition distribution in rape pollen, alkali concentration, pH value, time and temperature on the extraction percent of glutelins in rape pollen. The protein composition distribution of defatted and wall-broken rape pollen is albumin 34.66%, globulin 1.930%, prolamine 2.810%, glutelins 45.96%, complex protein 11.04%, and the major protein in rape pollen were albumin and glutelins. Through orthogonal test,the optimum conditions of extraction were obtained on the basis of single factor test, as follow: ratio of solid-liquid1:20, NaOH concentration 0.30%, pH value 11, time 75min, temperature 70℃.

Key words:  , rape pollen; glutelins; extraction;