基于MPN原理的TEMPO/TVC法检测食品中的细菌总数及其测量不确定度的评定

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 668-671.

基于MPN原理的TEMPO/TVC法检测食品中的细菌总数及其测量不确定度的评定

韩伟，顾鸣，金伟青

上海出入境检验检疫局；生物梅里埃中国公司

出版日期:2006-12-15 发布日期:2011-11-23

TEMPO/TVC,A Method of Total Viable Count in Foods Based on MPN Principle and the Evaluation of the Measure Uncertainty

HAN Wei, GU Ming, JIN Wei-Qing

1.Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China; 2.Bio-merieux China Co.Ltd., Shanghai 200135, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 细菌总数(也称需氧细菌数)的多少在一定程度上标志着食品卫生质量的优劣,是最常见的食品卫生指标之一。本文比较了传统的菌落计数方法和基于最大近似值(MPN)原理的TEMPO/TVC仪器法,对多个种类的190个食品样品进行了检测。实验数据的比对表明TEMPO/TVC法与菌落计数方法一致性程度较好(符合率97.4%,准确度和精确度无差异),测量不确定度相近,具有简便、快速、高效、人为误差较小的特点。

关键词: TEMPO/TVC法, MPN, 测量不确定度

Abstract: In this study, the TEMPO/TVC(Total viable count)method, a new automated enumeration based on MPN method, was compared to the plate count method by testing a variety of food products. The results of the two methods were compared for 190 samples. Good correlation was showed between the new method and the reference methods, the measure uncertainties of the two methods is also familiar, while TEMPO/TVC automated method is simpler, less time-consuming, more effective and more stable.

Key words: , TEMPO/TVC method； MPN method； measure uncertainty；

韩伟, 顾鸣, 金伟青. 基于MPN原理的TEMPO/TVC法检测食品中的细菌总数及其测量不确定度的评定[J]. 食品科学, 2006, 27(12): 668-671.

HAN Wei, GU Ming, JIN Wei-Qing. TEMPO/TVC,A Method of Total Viable Count in Foods Based on MPN Principle and the Evaluation of the Measure Uncertainty[J]. FOOD SCIENCE, 2006, 27(12): 668-671.

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