副溶血性弧菌的亚致死热损伤和复苏条件分析

doi:10.7506/spkx1002-6630-201321046

食品科学 ›› 2013, Vol. 34 ›› Issue (21): 227-232.doi: 10.7506/spkx1002-6630-201321046

副溶血性弧菌的亚致死热损伤和复苏条件分析

陈婵娟，宁喜斌*，丛健，盛骥春

上海海洋大学食品学院，上海水产品加工及贮藏工程技术研究中心，上海 201306

收稿日期:2012-10-21 修回日期:2013-09-30 出版日期:2013-11-15 发布日期:2013-10-28
通讯作者: 宁喜斌 E-mail:xbning@shou.edu.cn
基金资助:
上海市科委工程中心建设项目(11DZ2280300)；上海市教育委员会重点学科建设项目(J50704)

Sublethal Thermal Injury and Resuscitation Conditions of Vibrio parahaemolyticus

CHEN Chan-juan，NING Xi-bin*，CONG Jian，SHENG Ji-chun

Shanghai Engineering Research Center of Aquatic-product Processing and Preservation, College of Food Science and Technology,
Shanghai Ocean University, Shanghai 201306, China

Received:2012-10-21 Revised:2013-09-30 Online:2013-11-15 Published:2013-10-28
Contact: NING Xi-bin E-mail:xbning@shou.edu.cn

摘要/Abstract

摘要：

目的：研究不同温度条件下副溶血性弧菌的亚致死热损伤程度，最适宜的复苏条件以及复苏后细菌的生长特性。方法：运用传统的固体平板法对不同热处理条件下副溶血性弧菌VpBJ1.1997细胞的亚致死损伤率进行检测，然后通过液体法绘制细菌复苏生长曲线，并结合不同复苏时间下通过扫描电镜观察副溶血性弧菌的形态特征和复苏后亚致死损伤率的测定，最终确定准确的亚致死损伤和复苏条件。结果：副溶血性弧菌在45℃条件下处理时间10min，亚致死损伤率达到最高；35℃复苏3h后，损伤程度最低。结论：不同温度下的亚致死损伤率说明，副溶血性弧菌在30℃条件下培养可以降低亚致死损伤导致的漏检率。

关键词: 副溶血性弧菌, 亚致死损伤, 复苏

Abstract:

Objective: To study the degree of sublethal thermal injury in Vibrio parahaemolyticus, the optimum resuscitation
temperature of the sublethally injured strains and the growth characteristics of resuscitative strains. Methods: Firstly,
we detected the degress of sublethal injury in Vibrio parahaemolyticus BJ1.1997 at different temperatures by using
traditional solid agar methods. Then, resuscitative growth curves of sublethally injured Vibrio parahaemolyticus BJ1.1997
were established by using liquid cultivation method. The morphological characteristics of the sublethally injuries and
the resuscitative strains were observed by using SEM and the optimal conditions of sublethal injury and resuscitation
temperature of Vibrio parahaemolyticus BJ1.1997 were determined. Results: The highest extent of sublethal injury in Vibrio
parahaemolyticus BJ1.1997 was obtained after 10 min treatment at 45 ℃ as well as the lowest injury in strains resuscitated
at 35 ℃ for 3 h. Conclusion: According to the measurement of sublethal injury at different temperatures, the probability of
Vibrio parahaemolyticus false-negative rate at 30 ℃ can be reduced.

Key words: Vibrio parahaemolyticus, sublethal injury, resuscitation

中图分类号:

R155

陈婵娟，宁喜斌*，丛健，盛骥春. 副溶血性弧菌的亚致死热损伤和复苏条件分析[J]. 食品科学, 2013, 34(21): 227-232.

CHEN Chan-juan，NING Xi-bin*，CONG Jian，SHENG Ji-chun. Sublethal Thermal Injury and Resuscitation Conditions of Vibrio parahaemolyticus[J]. FOOD SCIENCE, 2013, 34(21): 227-232.

参考文献

[1]Su Y. C..Vibrio parahaemolyticus: a concern of seafood safety[J].Food Microbiol,2007,24(6):549-558

[2] Wu VCH. A review of microbial injury and recovery methods in food[J][J].Food microbiology,2008,25((6)):735-744

[3]Hartsell. S.The Longevity and Behavior of Pathogenic Bacteria in Frozen Foods: The Influence of Plating Media[J].American Journal of Public Health and the Nations Health,1951,41(9):1072-1077

[4]Hoefel D, Grooby W L, Monis P T, et al. .Enumeration of water-borne bacteria using viability assays and flow cytometry: a comparison to culture-based techniques[J].Journal of microbiological methods,2003,55(3):585-597

[5] Shintani H. Importance of considering injured microorganisms in sterilization validation[J].Biocontrol science,2006,11(3):91-

[6]Ray B., Hawkins S.M., H.C.R, et al. .Method for the detection of injured Vibrio parahaemolyticus in seafoods[J].Applied and environmental microbiology,1978,35(6):1121-1127

[7]Duan J., Liu C., S. Y.C., et al..Evaluation of a double layer agar plate for direct enumeration of Vibrio parahaemolyticus[J].Journal of food science,2006,71(2):M77-M82

[8]Wu VCH, Fung DYC, Kang DH, et al. .Evaluation of thin agar layer method for recovery of acid-injured foodborne pathogens[J].Journal of Food Protection,2001,64(7):1067-1071

[9]Kang D.H., S. GR. .Agar underlay method for recovery of sublethally heat-injured bacteria[J].Applied and environmental microbiology,1999,65(12):5334-5337

[10]Wu VCH, F. DYC.. Evaluation of thin agar layer method for recovery of heat‐injured foodborne pathogens[J].Journal of food science,2001,66(4):580-583

[11]Heinis, J., L. Beuchat, W. Jones, et al. .Growth of heat-injured Vibrio parahaemolyticus in media supplemented with various cations[J].Applied and environmental microbiology,1977,33(5):1079-1084

[12]Kang D.H., Siragusa GR.. .A rapid twofold dilution method for microbial enumeration and resuscitation of uninjured and sublethally injured bacteria[J].Letters in applied microbiology,2001,33(3):232-236

[13]钱天乐, 周逸卿, 邹珍友, 等. .微生物扫描电镜样品清洗方法的改进与固定干燥方法比较[J].安徽农业科学,2009,37(023):10886-10888

[14]Chiang, M.L., W.L. Ho, C.C. Chou., et al.. Response of Vibrio parahaemolyticus to ethanol shock[J].Food microbiology,2006,23(5):461-467

[15]Ye M., Huang Y., Neetoo H., et al. .Influence of growth conditions on pressure resistance of Vibrio parahaemolyticus in oysters and the optimization of postpressure treatment recovery conditions[J].Journal of Food Protection,2011,74(5):751-758

[16]刘代新, 宁喜斌, 张继伦. .响应面分析法优化副溶血性弧菌生长条件[J].微生物学通报,2008,35(2):306-310

[17]索标, 滕要辉, 史贤明, 等.. 食品中热损伤沙门氏菌选择性增菌及实时荧光聚合酶链式反应检测[J].食品科学,2012,33(10):223-227

[18]邱波, 姚斐, 姚怡, 等. .低温对常见致病弧菌菌种保存的影响[J].职业与健康,2003,19(9):53-54

[19]Busta, F. .Practical implications of injured microorganisms in food[J].Journal of Milk and Food Technology,1976,39(2):138-145

[20] 李志明. 食品卫生微生物检验学[M]. 北京: 化学工业出版, 2009: 49-52.

副溶血性弧菌的亚致死热损伤和复苏条件分析

Sublethal Thermal Injury and Resuscitation Conditions of Vibrio parahaemolyticus

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