食品科学 ›› 2018, Vol. 39 ›› Issue (6): 177-182.doi: 10.7506/spkx1002-6630-201806028

• 生物工程 • 上一篇    下一篇

胁迫生长条件下副溶血性弧菌的生物特性分析

高璐1,欧阳敏1,张辉2,饶胜其1,尹永祺1,杨振泉1,3,*,沈明君4   

  1. (1.扬州大学食品科学与工程学院,江苏?扬州 225127;2.江苏省农业科学院农产品质量安全与营养研究所,江苏?南京 210014;3.江苏省人兽共患病重点实验室,江苏?扬州 225127;4.扬州朝天歌农牧科技有限公司,江苏?扬州 225000)
  • 出版日期:2018-03-25 发布日期:2018-03-14
  • 基金资助:
    江苏省农业科技自主创新资金项目(CX(15)1012);江苏省人兽共患病重点实验室开放课题项目(R1508); 扬州市社会发展前瞻性研究项目(YZ2014177)

Biological Characteristics of Vibrio parahaemolyticus during Growth in Adverse Environment

GAO Lu1, OUYANG Min1, ZHANG Hui2, RAO Shengqi1, YIN Yongqi1, YANG Zhenquan1,3,*, SHEN Mingjun4   

  1. (1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China;2. Institute of Quality Safety and Nutrition of Agricultural Products, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 3. Jiangsu Key Laboratory of Zoonosis, Yangzhou 225127, China;4. Yangzhou Chaotiange Agriculture and Animal Husbandry Technology Co. Ltd., Yangzhou 225000, China)
  • Online:2018-03-25 Published:2018-03-14

摘要: 为了解副溶血性弧菌在不同环境应激条件下的生长情况,以副溶血性弧菌标准菌株(Vibrio parahaemolyticus,CICC21617)为研究对象,使其在低盐度(0.9?g/100?mL?NaCl)、低pH值(pH?5.0)和高温(50?℃)条件下适应性传代培养后,通过测定菌株生存能力、生物被膜生成能力、溶血素活力和胞外蛋白酶产量等指标分析胁迫条件下副溶血性弧菌的生物特性。结果显示,经过胁迫传代的菌株较原始菌株,其生存能力和生物被膜生成能力有所增强,而溶血素活力和胞外蛋白酶产量却明显下降,外膜蛋白的种类和表达量亦有所不同。结果说明副溶血性弧菌可能是通过改变自身生物学特性来适应外界的不利生存环境,且菌株经应激适应后对逆境生存环境的耐受性明显增强。

关键词: 副溶血性弧菌, 胁迫, 生长, 生物特性

Abstract: The objective of this study was to observe the growth characteristics of Vibiro parahaemolyticus under various environmental stresses. The survival, biofilm formation ability, hemolysin activity and extracellular protease production of V. parahaemolyticus (CICC21617) exposed to low salinity (0.9 g/100 mL NaCl), low pH (5.0) or high temperature (50 ℃) were investigated after 10 passages. It was found that the stressed and passaged strains had enhanced survival and biofilm formation ability and decreased hemolysin activity and showed significantly reduced extracellular protease production compared with the parental strain. The expression levels and types of low molecular weight outer membrane proteins of the stressed strains were also different between the parental and passaged strains. Thus, V. parahaemolyticus is capable of changing their own biological characteristics to adapt to adverse conditions. Furthermore, the tolerance of V. parahaemolyticus to environmental stress can be enhanced after adaptive training.

Key words: Vibrio parahaemolyticu, stress, growth, growth characteristics

中图分类号: