关键词: 马鹿角蛋白, 酶解物, 抗氧化, 抗疲劳, 免疫功能

Abstract:

The antioxidant, anti-fatigue and immuoregulatory effects of red deer (Cervus elaphus) antler protein and
hydrolysate prepared with both protamex and flavourzyme were investigated. For the determination of antioxidant effects,
pyrogallol auto-oxidation method, salicylate colorimetry and total reducing power assay were used. The in vivo antioxidant
potential of antler protein hydrolysate was analyzed by measuring SOD activity and MDA content in the serum of mice
receiving oral administration. Weight-loaded swimming test and blood lactic acid and liver glycogen assays were performed
to assess its anti-fatigue effect. Besides, the immunoregulatory effect of the hydrolysate in mice was examined by ConAinduced
mice spleen lymphocyte transformation and carbon clearance tests. Our results indicated that red deer antler protein
hydrolysate possessed scavenging activity against hydroxyl and superoxide anion radicals and improved total reducing
power compared to red deer antler protein. In addition, oral administration of this hydrolysate increased serum SOD activity,
reduced serum MDA content, obviously prolonged swimming time, decreased blood lactic acid and liver glycogen levels,
and remarkably enhanced the immune function of mice. From these observations, we concluded that red deer antler protein
hydrolysate has significantly stronger antioxidant, anti-fatigue and immuoregulatory effects than red deer antler protein.

Key words: red deer antler protein, hydrolysate, antioxidant activity, anti-fatigue activity, immuneregulatory function