关键词: B族维生素, 婴幼儿配方乳粉, 超高压液相色谱质谱法

Abstract: Objective Establish a can simultaneously determination of 11 kinds of B vitamins in infant formula by ultra-pressure liquid-chromatography tandem mass spectrometry (UPLC-MS/MS).Methods The sample used 0.1 % formic acid to dissolved for extraction, zinc acetate precipitate protein. Then the filtrate was separated by an ACQUITY UPLC HSS T3 column, and gradient elution was performed with 0.1 % formic acid and acetonitrile as the mobile phase. Mass spectral acquisition was done in the positive ion mode by applying multiple reaction monitoring, analysis time 12min. Results The 11 kinds of B vitamins in lineal range had got high correlation coefficients(R2>0.99) .The spiked recovery rates were determined between 85%~110%,accuracy between 1.03 %~6.75 %. Vitamin B1, Vitamin B2,Nicotinic acid, niacinamide, pantothenic acid, pyridoxal, pyridoxine, pyridoxamine, folic acid, Vitamin H and vitamin B12 of the lower limit of quantitation (LOQ) were 40.0, 40.0, 30.0, 30.0, 50.0, 0.9, 1.2, 1.2, 1.2, 2.0, 2.0μg/100g. Comparison with national standards, the limit of quantification increase by 7.7% to 60%.Conclusion The method has high sensitivity, simple operation，fast analysis short time characteristics, but also has good precision and accuracy that can be applied to infant formula in 11 kinds of B vitamins detection.

Key words: B vitamins, Infant formula milk powder, Ultra Pressure Liquid Chromatography-Mass spectrometry/Mass spectrometry