关键词: SSR荧光标记, 绿豆, 遗传多样性分析, 指纹图谱构建

Abstract: Objective: The aim of this study was to analyze the genetic diversity of mungbean varieties from Inner Mongolia region and to establish a simple sequence repeat (SSR) fingerprinting database for providing scientific evidence to explore the genetic background of mungbean germplasms and authenticate mungbean varieties. Methods: A total of 92 mungbean varieties from Inner Mongolia area were taken to establish their genetic diversity and DNA fingerprint maps by using 10 pairs of SSR primers. Results: A total of 58 alleles were detected, and each pair of primers detected 3–10 alleles with an average of 5.8. The genetic diversity coefficients among the 92 mungbean varieties varied from 0.033 4–1.000 0 with an average of 0.46. Unweighted pair-group method with arithmetic means (UPGMA) clustering analysis showed that these mungbean varieties were clustered into 2 groups at theof genetic similarity coefficient of 0.294 4. SSR fingerprinting database and molecular identification of the 92 mungbean cultivars were established, and it was found that most of the tested varieties, with different SSR fingerprints, which could be molecularly discriminated from each other, could serve as cultivars-specific patterns and as an important basis for cultivar identification. Conclusions: There was a high level of genetic diversity among mungbean germplasms from Inner Mongolia, but a certain degree of inbreeding also existed. Therefore, further efforts are needed to develop new mungbean breeding materials. SSR fingerprinting and molecular identification using fluorescent labeled SSR marker technique is of great significance for genetic authentication, identity validation, traceability management and origin protection of mungbean varieties.

Key words: fluorescent labeled SSR markers, mungbean varieties, genetic diversity analysis, fingerprint construction