食品科学 ›› 2012, Vol. 33 ›› Issue (8): 39-42.doi: 10.7506/spkx1002-6630-201208009

• 工艺技术 • 上一篇    下一篇

超临界CO2萃取黑莓籽油及其成分分析

周鸣谦,刘云鹤,林春梅   

  1. 淮海工学院食品科学与工程学院
  • 出版日期:2012-04-25 发布日期:2012-03-31
  • 基金资助:
    江苏省产学研联合创新资金计划项目(BY2010127);连云港市农业攻关计划项目(CN0905)

Supercritical Carbon Dioxide Extraction and Fatty Acid Composition Analysis of Blackberry Seed Oil

ZHOU Ming-qian,LIU Yun-he,LIN Chun-mei   

  1. (College of Food Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China)
  • Online:2012-04-25 Published:2012-03-31

摘要: 目的:以黑莓籽为原料,用超临界CO2萃取法提取黑莓籽油,并测定其成分。方法:采用超临界CO2萃取方法提取黑莓籽油,通过正交试验对影响提取过程的参数进行优化,确定黑莓籽油提取的最佳工艺条件,并用气相色谱-质谱法分析黑莓籽油的脂肪酸组成。结果:超临界CO2流体萃取黑莓籽油的最佳工艺条件为萃取温度35℃、萃取压力30MPa、分离压力12MPa、分离温度55℃,此条件下黑莓籽油的得率达16.10%,其脂肪酸组成为软脂酸5.38%、硬脂酸3.53%、油酸13.70%、亚油酸60.48%、亚麻酸11.16%,总不饱和脂肪酸含量85.34%。

关键词: 超临界CO2, 萃取, 黑莓籽油, 气相色谱-质谱, 脂肪酸

Abstract: Objective: To extract blackberry seed oil from blackberry seeds with supercritical carbon dioxide and determine its fatty acid composition. Methods: Operating parameters that influence the extraction of blackberry seed oil were optimized using an orthogonal array design. The fatty acid composition of blackberry seed oil was analyzed by gas chromatography-mass spectrometry (GC-MS). Results: The optimal extraction conditions were extraction temperature of 35 ℃, extraction pressure of 12 MPa, separation pressure of 12 MPa and separation temperature of 55 ℃. Under thess conditions, the yield of blackberry seed oil was up to 16.10%. The major fatty acids were palmic acid (5.38%), stearic acid (3.53%), oleic acid (13.70%), linoleic acid (60.48%), gamma-linolenic acid (11.16%) and total unsaturated fatty acid (85.34%).

Key words: supercritical carbon dioxide, extraction, blackberry seed oil, gas chromatography-mass spectrometry (GC-MS), fatty acid

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