苦荞茶多糖诱导人肺腺癌A549细胞凋亡的线粒体机制研究

摘要/Abstract

摘要： 目的：从线粒体调控机制的角度探讨苦荞茶多糖(tartary buckwheat tea polysaccharide，TBTP)对肺癌细胞凋亡的影响。方法：应用水提醇沉法结合超声辅助技术提取的TBTP处理人肺腺癌A549细胞，分为空白对照组和不同浓度TBTP处理组。采用MTT法和流式细胞术检测细胞增殖和凋亡水平，采用DHE和Mito SOX染色检测细胞和线粒体活性氧簇的水平，采用JC-1染色检测线粒体膜电位变化，采用Western blot法检测凋亡相关蛋白Bax、Bcl-2、剪切型caspase-3、细胞色素C的水平。结果：TBTP呈剂量和时间依赖性抑制A549细胞增殖。TBTP处理A549细胞24h时，TBTP呈剂量依赖地促进细胞凋亡。TBTP处理能够显著增加A549细胞和线粒体内活性氧簇含量，降低线粒体膜电位水平；同时，TBTP能够增加促凋亡蛋白Bax和Cleaved caspase-3的表达、抑制抗凋亡蛋白Bcl-2的表达，促进A549细胞线粒体细胞色素C外流。结论：TBTP能够促进活性氧簇生成、降低线粒体膜电位，进而抑制人肺腺癌A549细胞增殖、促进细胞凋亡。

关键词: 苦荞茶, 多糖, 肺癌, 细胞凋亡

Abstract: Objective: To investigate the effect of TBTP on the apoptosis of A549 human lung cancer cells and its underlying mechanisms. Methods: TBTP was obtained by water extraction. MTT assay was used to detect the influence of TBTP on the proliferation of A549 cells. And flow cytometry was applied to determine the effect of TBTP on the cell apoptosis of A549 cells. The production of reactive oxygen species (ROS) was assessed by DHE and Mito SOX Red. The change of mitochondrial membrane potential was assessed by JC-1. Meanwhile, western blot analysis was used to evaluate the expression of Bax, Bcl-2, Cleaved caspase-3 and cytochrome C. Results: MTT assay showed a significant inhibitory effect of TBTP on A549 cells proliferation in a dose- and time-dependent manner. And after 24 hours’ cultivation, TBTP also promoted A549 cells apoptosis in a dose-dependent manner. TBTP treatment promoted mitochondrial ROS production and oxidative stress in A549 cells. The mitochondrial membrane potential of A549 cells also decreased after TBTP administration, accompanied with increased cytochrome C release from mitochondria. Furthermore, TBTP treatment up-regulated the expression of Bax and Cleaved caspase-3, and decreased Bcl-2 expression. Conclusions: TBTP exerts a novel anti-proliferative effect through induction of mitochondria-mediated apoptosis in A549 cells.

Key words: tartary buckwheat tea, polysaccharide, lung cancer, apoptosis

中图分类号:

TS201.4

张敏杨二万杨兴斌董玲杨红燕. 苦荞茶多糖诱导人肺腺癌A549细胞凋亡的线粒体机制研究[J]. 食品科学, 0, (): 0-0.

Hong-Yan YANG. Tartary Buckwheat Tea Polysaccharide induces mitochondria-mediated apoptosis in A549 human lung cancer cells[J]. FOOD SCIENCE, 0, (): 0-0.

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