浒苔多糖对脂多糖诱导小鼠肠道屏障功能及肠道微生物组成的影响

doi:10.7506/spkx1002-6630-20230128-175

食品科学 ›› 2024, Vol. 45 ›› Issue (3): 34-41.doi: 10.7506/spkx1002-6630-20230128-175

浒苔多糖对脂多糖诱导小鼠肠道屏障功能及肠道微生物组成的影响

张玉梅，谢春艳，吴信

（1.中国科学院亚热带农业生态研究所，亚热带农业生态过程重点实验室，湖南长沙 410125；2.天津市农业科学院畜牧兽医研究所，天津 300381；3.中国科学院天津工业生物技术研究所，天津 300308）

出版日期:2024-02-15 发布日期:2024-03-06
基金资助:
国家自然科学基金青年科学基金项目（31902196）；中国博士后科学基金第66批面上项目（2019M662273）

Effect of Enteromorpha prolifera Polysaccharide on Lipopolysaccharide-Induced Intestinal Barrier Injury and Intestinal Microbial Composition in Mice

ZHANG Yumei, XIE Chunyan, WU Xin

(1. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; 2. Institute of Animal Science and Veterinary, Tianjin Academy of Agriculture Sciences, Tianjin 300381, China; 3. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China)

Online:2024-02-15 Published:2024-03-06

摘要/Abstract

摘要： 目的：研究浒苔多糖（Enteromorpha prolifera polysaccharide，EP）对脂多糖（lipopolysaccharide，LPS）诱导小鼠肠道屏障功能及微生物组成的影响。方法：选取8 周龄C57BL/6J雄性小鼠48 只，随机分为4 组，每组12 只，分别为空白对照（Ctrl）组、LPS组、EP组、EP＋LPS组，Ctrl和LPS组小鼠饲喂基础日粮，EP和EP＋LPS组饲喂含600 mg/kg EP的日粮，28 d后，LPS和EP＋LPS组小鼠按0.5 mg/kg mb腹腔注射200 µL LPS，Ctrl和EP组小鼠腹腔注射相同剂量的生理盐水。结果：EP处理4 周对LPS诱导的小鼠肝脏损伤和脾脏肿大没有显著影响；EP处理能显著降低LPS诱导小鼠的血清中髓过氧化物酶（P＜0.05）和二胺氧化酶（P＜0.01）的含量，显著提高结肠白细胞介素（interleukin，IL）-1β的基因表达（P＜0.05），显著降低结肠一氧化氮合成酶（inducible nitric oxide synthase，iNOS）的基因表达（P＜0.05）和Toll样受体4的蛋白表达；在门水平上，EP处理能极显著提高LPS诱导小鼠Verrucomicrobiota的丰度（P＜0.01），在属水平上，显著降低Alloprevotella、Bacteroides和unclassified_o_Bacteroidales的丰度（P＜0.05），显著提高Lachnospiraceae_NK4A136_group、Anaerostipes和Akkermansia的丰度（P＜0.05）。结论：EP可以预防LPS刺激引起的肠道屏障功能受损，并可通过调节TLR4信号通路相关基因的表达和肠道微生物的组成减缓小鼠的肠道屏障损伤。

关键词: 浒苔多糖；脂多糖；炎症；肠道屏障；肠道微生物

Abstract: Objective: This study was conducted to investigate the effect of Enteromorpha prolifera polysaccharide (EP) on lipopolysaccharide (LPS)-induced intestinal barrier injury and the intestinal microbial composition in mice. Methods: Forty-eight 8-week-old C57BL/6J male mice were selected and randomly divided into four groups (12 mice/group): control (Ctrl), LPS, EP, and EP + LPS. The mice in the Ctrl and LPS groups were provided with a basal diet, and those in the EP and EP + LPS groups were given a basal diet supplemented with 600 mg/kg EP. After feeding for 28 days, the mice in the LPS and EP + LPS groups were injected intraperitoneally with 200 µL of LPS solution at a dose of 0.5 mg/kg body mass, and those in the Ctrl and EP groups were given the same dose of normal saline. Results: After 4 weeks of EP treatment, there was no significant change in LPS-induced liver damage or splenomegaly in mice (P > 0.05). Compared with the LPS group, EP treatment significantly reduced the serum levels of myeloperoxidase (MPO) (P < 0.05) and diamine oxidase (DAO) (P < 0.01), elevated the colonic gene expression of interleukin-1β (IL-1β) (P < 0.05), and decreased the colonic gene expression of inducible nitric oxide synthase (iNOS) (P < 0.05) and colonic Toll-like receptor 4 (TLR4) expression (P < 0.05). At the phylum level, EP treatment significantly increased the abundance of Verrucomicrobiota in mice with LPS-induced intestinal barrier injury (P < 0.01); at the genus level, it significantly decreased the abundance of Alloprevotella, Bacteroides and unclassified_o_Bacteroidales (P < 0.05) and increased the abundance of Lachnospiraceae_NK4A136_group, Anaerostipes and Akkermansia (P < 0.05). Conclusion: EP not only can prevent LPS-induced impairment of intestinal barrier function, but also can alleviate intestinal barrier damage in mice by regulating the expression of genes related to the TLR4 signaling pathway and the composition of the intestinal microbiota.

Key words: Enteromorpha prolifera polysaccharide; lipopolysaccharide; inflammation; intestinal barrier; gut microbes

中图分类号:

TS201.4

张玉梅，谢春艳，吴信. 浒苔多糖对脂多糖诱导小鼠肠道屏障功能及肠道微生物组成的影响[J]. 食品科学, 2024, 45(3): 34-41.

ZHANG Yumei, XIE Chunyan, WU Xin. Effect of Enteromorpha prolifera Polysaccharide on Lipopolysaccharide-Induced Intestinal Barrier Injury and Intestinal Microbial Composition in Mice[J]. FOOD SCIENCE, 2024, 45(3): 34-41.

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浒苔多糖对脂多糖诱导小鼠肠道屏障功能及肠道微生物组成的影响

Effect of Enteromorpha prolifera Polysaccharide on Lipopolysaccharide-Induced Intestinal Barrier Injury and Intestinal Microbial Composition in Mice

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