大蒜多糖通过核因子-κB通路改善代谢相关脂肪性肝病小鼠肝脏损伤

doi:10.7506/spkx1002-6630-20230322-226

食品科学 ›› 2024, Vol. 45 ›› Issue (3): 53-60.doi: 10.7506/spkx1002-6630-20230322-226

大蒜多糖通过核因子-κB通路改善代谢相关脂肪性肝病小鼠肝脏损伤

刘杰，玉王宁，王成海，李沙，程立媛，张伟

（1.河北农业大学食品科技学院，河北保定 071001；2.河北工程大学生命科学与食品工程学院，河北邯郸 056038；3.河北工程大学医学院，河北邯郸 056038；4.河北工程大学附属医院，河北省血液净化应用基础研究重点实验室，河北邯郸 056002；5.河北工程大学分析测试实验中心，河北邯郸 056038；6.河北农业大学生命科学学院，河北保定 071001）

出版日期:2024-02-15 发布日期:2024-03-06
基金资助:
国家自然科学基金青年科学基金项目（81800549）；河北省自然科学基金青年科学基金项目（H2020402002）；邯郸市科学技术研究与发展计划项目（21422073228；21422083266）

Garlic Polysaccharides Ameliorate Liver Injury in Mice Metabolic-Associated Fatty Liver Disease by Regulating the Nuclear Factor-κB Signaling Pathway

LIU Jie, YU Wangning, WANG Chenghai, LI Sha, CHENG Liyuan, ZHANG Wei

(1. College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China; 2. School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, China; 3. Medical College, Hebei University of Engineering, Handan 056038, China; 4. Hebei Key Laboratory of Applied Basic Research of Blood Purification, Affiliated Hospital of Hebei Engineering University, Handan 056002, China; 5. Analysis and Testing Laboratory Center, Hebei University of Engineering, Handan 056038, China; 6. College of Life Sciences, Hebei Agricultural University, Baoding 071001, China)

Online:2024-02-15 Published:2024-03-06

摘要/Abstract

摘要： 目的：探讨大蒜多糖对小鼠代谢相关脂肪性肝病（metabolic-associated fatty liver disease，MAFLD）的保护作用。方法：50 只雄性C57BL/6小鼠随机等分为5 组，分别为正常组，模型组，大蒜多糖低、中、高剂量组（250、1 000、3 000 mg/kg mb），饲喂28 d后采样，分别检测病理形态、血清和肝组织的生化指标，定量分析Hmox1、Cat、Gpx1、Tnf、Ccl2、Cxcl2、Cxcl10、IL-1a、IL-1b、Fasn、Acaca、Cpt1a、Acox、Cd36、Fabp1、Apob和Mttp等基因的表达情况。结果：与模型组相比，中、高剂量组大蒜多糖对MAFLD有比较明显的缓解作用，病理形态基本恢复正常，肝组织丙二醛和超氧化物歧化酶趋近正常组水平，Hmox1、Tnf、Ccl2、Cxcl2、Cxcl10、Cpt1a和Acox等基因的表达水平得到显著回复。结论：大蒜多糖的干预基本消除了MAFLD导致的肝损伤，中剂量组效果显著且添加量适中，作用机制可能是通过核因子κB信号通路调控脂质代谢和炎性基因表达，减少脂质沉积和脂质过氧化。

关键词: 大蒜多糖；代谢相关脂肪性肝病；炎症反应；脂质沉积

Abstract: Objective: To investigate the protective effect of garlic polysaccharides against metabolic-associated fatty liver disease (MAFLD) in mice. Methods: Totally 50 male C57BL/6 mice were randomly equally divided into five groups, namely, normal, model, low-, medium-, and high-dose garlic polysaccharides (250, 1 000, and 3 000 mg/kg mb, respectively). After 28 days of feeding, serum and liver samples were taken for pathomorphological examination, biochemical assays, and quantitative analysis of the expression of the genes Hmox1, Cat, Gpx1, Tnf, Ccl2, Cxcl2, Cxcl10, IL-1a, IL-1b, Fasn, Acaca, Cpt1a, Acox, Cd36, Fabp1, Apob and Mttp. Results: Compared with the model group, garlic polysaccharides at the medium and high doses significantly alleviated MAFLD, restoring the pathological morphology to normal, bringing the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in liver tissue back to near-normal levels, and obviously restoring the expression of Hmox1, Tnf, Ccl2, Cxcl2, Cxcl10, Cpt1a, and Acox to normal levels. Conclusion: Intervention of garlic polysaccharides, especially at the medium dose, can basically eliminate liver damage caused by MAFLD. The underlying mechanism may be related to the regulation of lipid metabolism and inflammatory gene expression through suppressing the nuclear factor-κB signaling pathway, thereby reducing lipid deposition and lipid peroxidation.

Key words: garlic polysaccharides; metabolic associated fatty liver disease; inflammatory reaction; lipidosis

中图分类号:

TS201.4

刘杰，玉王宁，王成海，李沙，程立媛，张伟. 大蒜多糖通过核因子-κB通路改善代谢相关脂肪性肝病小鼠肝脏损伤[J]. 食品科学, 2024, 45(3): 53-60.

LIU Jie, YU Wangning, WANG Chenghai, LI Sha, CHENG Liyuan, ZHANG Wei. Garlic Polysaccharides Ameliorate Liver Injury in Mice Metabolic-Associated Fatty Liver Disease by Regulating the Nuclear Factor-κB Signaling Pathway[J]. FOOD SCIENCE, 2024, 45(3): 53-60.

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大蒜多糖通过核因子-κB通路改善代谢相关脂肪性肝病小鼠肝脏损伤

Garlic Polysaccharides Ameliorate Liver Injury in Mice Metabolic-Associated Fatty Liver Disease by Regulating the Nuclear Factor-κB Signaling Pathway

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