刺梨多糖对非酒精性脂肪肝小鼠回肠粘膜屏障功能的影响

摘要/Abstract

摘要： 为了研究刺梨多糖（Rosa roxburghii Tratt polysaccharide, RTFP）对非酒精性脂肪肝（nonalcoholic fatty liver disease, NAFLD）诱导的小鼠回肠肠道粘膜屏障功能障碍和肠道炎症的改善作用，以及肠道菌群与其活性之间的潜在联系。本研究采用高脂肪饮食（high fat diet, HFD）喂养小鼠构建NAFLD小鼠模型，然后灌胃RTFP。干预7周后，结果表明，RTFP可显著降低NAFLD诱导的小鼠回肠炎症因子和氧化应激水平，改善脂质代谢紊乱状况。微观组织病理学观察发现，RTFP可以使回肠结构形态趋向于正常水平，减少隐窝病变，保护肠道屏障。通过16S rRNA高通量测序评估各处理组小鼠粪便微生物群的变化，发现RTFP可调节NAFLD小鼠的肠道菌群的多样性和组成，显著降低厚壁菌门（Firmicutes）与拟杆菌门（Bacteroidetes）的比例，提高有益菌群的丰度，降低病原菌群的丰度。这些结果表明RTFP可以作为一种益生元调节肠道菌群，改善NAFLD模型小鼠的肠道微生态，减缓肠道屏障功能障碍，维持肠道正常功能。

关键词: 刺梨多糖, 非酒精性脂肪肝, 高脂饮食, 肠道屏障障碍, 肠道微生物

Abstract: To investigate the ameliorative effects of Rosa roxburghii Tratt polysaccharide (RTFP) on ileal intestinal mucosal barrier dysfunction and intestinal inflammation in mice induced by non-alcoholic fatty liver disease (NAFLD), and the potential association between intestinal flora and its activity. In this study, NAFLD mouse model was established by feeding mice with high fat diet (HFD), and then RTFP was intragastric. After 7 weeks of intervention, RTFP significantly reduced the levels of inflammatory factors and oxidative stress in ileum of NAFLD-induced mice, and improved lipid metabolism disorders. Microscopic histopathological observations showed that RTFP could normalize the ileum structure, reduce crypt lesions and protect the intestinal barrier. The changes of fecal microbiota of mice in each treatment group were evaluated by 16S rRNA high-throughput sequencing. It was found that RTFP could regulate the diversity and composition of intestinal flora of NAFLD mice, significantly reduce the proportion of Firmicutes and Bacteroidetes, improve the abundance of beneficial flora and reduce the abundance of pathogenic flora. These results suggest that RTFP can be used as a prebiotic to regulate intestinal flora, improve intestinal microecology, reduce intestinal barrier dysfunction and maintain normal intestinal function in NAFLD model mice.

Key words: Rosa roxburghii Tratt polysaccharide, nonalcoholic fatty liver disease, high-fat diet, intestinal barrier disorder, intestinal microorganisms

中图分类号:

TS201.1

张潘汪磊陈洁许飞. 刺梨多糖对非酒精性脂肪肝小鼠回肠粘膜屏障功能的影响[J]. 食品科学, 0, (): 0-0.

Pan ZHANG Fei XU. Effects of Rosa roxburghii Tratt polysaccharide on ileal mucosal barrier function in mice with non-alcoholic fatty liver[J]. FOOD SCIENCE, 0, (): 0-0.

参考文献

[1]Paradies G, Paradies V, Ruggiero F M, et al.Oxidative stress,cardiolipin and mitochondrial dysfunction in nonalcoholic fatty liver disease[J].World Journal of Gastroenterology, 2014, 20(39):14205-14218
[2]Marcuccilli M, Chonchol M.NAFLD and chronic kidney disease[J].International Journal of Molecular Sciences, 2016, 17(4):562-
[3]Wiest R, Albillos A, Trauner M, et al.Targeting the gut-liver axis in liver disease[J].Journal of Hepatology, 2017, 67(5):1084-1103
[4]刘勤, 牛春燕.由“二次打击”到“多重打击”: 发病机制的演变带给非酒精性脂肪性肝病的治疗启示[J].世界华人消化杂志, 2019, 27(19):1171-1178
[5]Guarner F, Malagelada J-R.Gut flora in health and disease[J].The Lancet, 2003, 361(9356):512-519
[6]Rabiu BA, Gibson GR.Carbohydrates: a limit on bacterial diversity within the colon[J]. Biological Reviews of the Cambridge Philosophical Society, 2002, 77(3), 443–453. DOI:10.1017/s1464793102005961.[J].Biological Reviews of the Cambridge Philosophical Society, 2002, 77(3):443-453
[7]李金海.异功泽泻汤治疗非酒精性脂肪性肝炎例[J].河北中医, 2012, 34(6):833-834
[8]徐小妹，林文津，张亚敏，等.泽泻汤降脂作用与肠道微生态的相关性探讨[J].中国实验方剂学杂志, 2017, 23(3):116-121
[9]Jing N, Liu X, Jin M, et al.Fubrick tea attenuates high-fat diet induced fat deposition and metabolic disorder by regulating gut microbiota and caffeine metabolism[J].Food & Function, 2020, 11(8):6971-6986
[10]Wang L, Zhang B, Xiao J, et al.Physicochemical, functional, and biological properties of water-soluble polysaccharides from Rosa roxburghii Tratt fruit[J]. Food Chemistry, 2018, 249: 127–135. DOI:10.1016/j.foodchem.2018.01.011.[J].Food Chemistry, 2018, 249:127-135
[11]Yu L M, Fang N, Yang X S, et al.Effects of Rosa roxburghii Extract on Proliferation and Differentiation in Human Hepatoma SMMC-7721 Cells and CD34+ Haematopoietic Cells[J].Journal of Health Science, 2007, 53(1):10-15
[12]Wang L, Li C, Huang Q, et al.Polysaccharide from Rosa roxburghii Tratt fruit attenuates hyperglycemia and hyperlipidemia and regulates colon microbiota in diabetic db/db mice[J], Journal of Agricultural and Food Chemistry, 2019, 68(1): 147-159. DOI:10.1021/acs.jafc.9b06247.[J].Journal of Agricultural and Food Chemistry, 2019, 68(1):147-159
[13]汪磊.刺梨多糖的分离纯化、降血糖作用及其对肠道微生态的影响[D]. 广州: 华南理工大学，2019: 108-119.
[14]李宏睿, 张德琴, 金晓蕾, 等.营养性肥胖大鼠模型肠道菌群的微生物多样性分析[J].中国比较医学杂志, 2020, 30(11):1-9
[15]Badman M K, Flier J S.The Gut and Energy Balance: Visceral Allies in the Obesity Wars[J].Science, 2005, 307(5717):1909-1914
[16]Zhang S, Zhang Y, Ahsan MZ, et al.Atorvastatin Attenuates Cold-Induced Hypertension by Preventing Gut Barrier Injury[J].Journal of Cardiovascular Pharmacology, 2019, 74(2):143-151
[17]李超.脂多糖对小鼠肠道黏膜上皮屏障形态结构与防御功能的影响[D]. 咸阳：西北农林科技大学, 2021: 5-12.
[18]Maurizi G, Della Guardia L, Maurizi A, et al.A. Adipocytes properties and crosstalk with immune system in obesity-related inflammation[J]. Journal of Cellular Physiology, 2018, 233(1), 88–97. DOI:10.1002/jcp.25855.[J].Journal of Cellular Physiology, 2018, 233(1):88-97
[19]Sang T, Guo C, Guo D, et al.Suppression of obesity and inflammation by polysaccharide from sporoderm-broken spore of Ganoderma lucidum via gut microbiota regulation [J]. Carbohydrate Polymers, 2021, 256: 117594. DOI:10.1016/j.carbpol.2020.117594.[J].Carbohydrate Polymers, 2021, 256:117549-
[20]李成，孔晓雪，余炬波，等.基于高通量测序分析蟹糊微生物菌群多样性[J].食品科学, 2020, 4(4):134-139
[21]Li S, Qi Y, Chen L, et al.Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea[J]. International Journal of Biological Macromolecules, 2019, 124: 931–937. DOI:10.1016/j.ijbiomac.2018.11.271.[J].International Journal of Biological Macromolecules, 2019, 124:931-937
[22]Stojanov S, Berlec A, ?trukelj B.The Influence of Probiotics on the FirmicutesBacteroidetes Ratio in the Treatment of Obesity and Inflammatory Bowel disease[J].Microorganisms, 2020, 8(11):1715-
[23]Chang C J, Lin C S, Lu C C, et al.Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota[J].Nature Communications, 2015, 6(1):7489-
[24]Halawa M R, El-Salam M A, Mostafa B M, et al.The gut microbiome,Lactobacillus acidophilus; relation with type 2 diabates mellitus[J].Current Diabetes Reviews, 2019, 15(6):480-485
[25]Huang W C, Wei C C, Huang C C, et al.The beneficial effects of Lactobacillus plantarum PS128 on high-intensity,exercise-induced oxidative stress,inflammation,and performance in triathletes[J].Nutrients, 2019, 11(2):1-13
[26]Schepper J D, Collins F L, Rios-Arce N D, et al.Probiotic Lactobacillus reuteri prevents postantibiotic bone loss by reducing intestinal dysbiosis and preventing barrier disruption[J].Journal of Bone and Mineral Research, 2019, 18(1):1-18
[27]Coqueiro A Y, Raizel R, Bonvini A, et al.Probiotics for inflammatory bowel diseases: a promising adjuvant treatment[J].International Journal of Food Sciences and Nutrition, 2019, 70(1):20-29
[28]Zeng H, Ishaq S L, Zhao F Q, et al.Colonic inflammation accompanies an increase of β-catenin signaling and Lachnospiraceae /Streptococcaceae bacteria in the hind gut of high-fat diet-fed mice[J]. The Journal of Nutritional Biochemistry, 2016, 35: 30-36. DOI: 10.1016/j.jnutbio.2016.05.015.[J].The Journal of Nutritional Biochemistry, 2016, 35:30-36
[29]Cui H X, Hu Y N, Li J W, et al.Hypoglycemic mechanism of the berberine organic acid salt under the synergistic effect of intestinal flora and oxidative stress[J]. Oxidative Medicine and Cellular Longevity, 2018, 2018: 1-13. DOI:10.1155/2018/8930374.[J].Oxidative Medicine and Cellular Longevity, 2018, 2018:1-13
[30]何丛.幽门螺杆菌感染对代谢综合征的影响及其与肠道菌群的相关性研究[D]. 南昌: 南昌大学, 2016: 1-4.
[31]Schneeberger M, Everard A, Gómez-Valadés A G, et al.Akkermansia muciniphila inversely correlates with the onset of inflammation,altered adipose tissue metabolism and metabolic disorders during obesity in mice[J].Scientific Reports, 2015, 5(1):2116-2130
[32]Everard A, Belzer C, Geurts L, et al.Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity[J].Proceedings of the National Academy of Sciences, 2013, 110(22):9066-9071
[33]Wang J H, Bose S, Kim, H G, et al.Fermented Rhizoma Atractylodis Macrocephalae alleviates high fat diet-induced obesity in association with regulation of intestinal permeability and microbiota in rats[J].Scientific reports, 2015, 5(1):8391-
[34]Mu H, Zhou Q, Yang R, et al.Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice[J]. Frontiers in Microbiology, 2020, 11: 585066. DOI:10.3389/fmicb.2020.585066.[J].Frontiers in Microbiology, 2020, 11:585066-
[35]Chen G, Xie M, Dai Z, et al.Kudingcha and fuzhuan brick tea prevent obesity and modulate gut microbiota in high-fat diet fed mice, Molecular Nutrition & Food Research, 2018, 62(6), 1700485. DOI:10.1002/mnfr.201700485.[J].Molecular Nutrition & Food Research, 2018, 62(6):1700485-