关键词: 高效液相色谱法, 动物组织, 安眠酮, 固相萃取

Abstract:

A high performance liquid chromatographic method was established to determine the content of methaqualone residues in pork. Sample extraction was carried out using dichloromethane as the extraction solvent, followed by clean-up on a solid-phase extraction column and dillution to a certain volum with the mobile phase composed of acetonitrile and 25 mmol/L ammonium acetate before injection onto HPLC system. The detection wavelength was 226 nm and the mobile phase flow rate was 1.0 mL/min. The calibration curve of the developed method was as follows: Y = 6.90 × 104X +3.71 × 103, with a correlation coefficient of 0.999, which exhibited good linearity over the concentration range from 0.020 to 0.20 mg/kg. The detection limit was 0.020 mg/kg, and the inter- and intra-laboratory average spike recovery rates of methaqualone at three levels were between 70% and 110%. This method has the benefits of simplicity, rapidity, high sensitivity, convenience and good feasibility and most suitable for the determination of methaqualone residues in pork.

Key words: high performance liquid chromatography, pork, methaqualone, SPE