FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (24): 272-277.doi: 10.7506/spkx1002-6630-201724044

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Uncertainty Evaluation of for Determining Inorganic Mercury, Methyl Mercury and Ethyl Mercury in Boletus by High Performance Liquid Chromatography Combined with Atomic Fluorescence Spectrometry

MAO Yongyang, YANG Tong, SU Tao, LI Zhigao, TIAN Jinlan   

  1. (1. Food Inspection and Testing Institute of Dali, Dali 671000, China; 2. Erhai Lake Conservation and Management Bureau of Dali, Dali 671000, China)
  • Online:2017-12-25 Published:2017-12-07

Abstract: In this study, we identified the main sources of uncertainty by mathematical modeling and calculated the main uncertainty components and combined and expanded uncertainties for the determination of inorganic mercury, methyl mercury and ethyl mercury in Boletus by high performance liquid chromatography combined with atomic fluorescence spectrometry (HPLC-AFS). The results showed that the major factors of uncertainty were concentration, instrumental measurement, sample heterogeneity, and recovery. Moreover, the curve fitting was the major factor causing the uncertainty for concentration. This study may provide a guideline for uncertainty evaluation for determining inorganic mercury, methyl mercury and ethyl mercury in edible fungus by HPLC-AFS.

Key words: high performance liquid chromatography combined with atomic fluorescence spectrometry, mercury specie, uncertainty, Boletus

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